Phosphoserine phosphatase Antibody (3A5) - Azide and BSA Free

Western Blot: Phosphoserine phosphatase Antibody (3A5) [H00005723-M01] - Analysis of PSPH expression in transfected 293T cell line by PSPH monoclonal antibody (M01), clone 3A5.Lane 1: PSPH transfected lysate(25 KDa).Lane 2: Non-transfected lysate.

Western Blot: Phosphoserine phosphatase Antibody (3A5) [H00005723-M01] - PSPH monoclonal antibody (M01), clone 3A5. Analysis of PSPH expression in human placenta.

Sandwich ELISA: Phosphoserine phosphatase Antibody (3A5) [H00005723-M01] - Detection limit for recombinant GST tagged PSPH is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Phosphoserine phosphatase Antibody (3A5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PSPH (AAH63614, 1 a.a. ~ 225 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVSHSELRKLFYSADAVCFDVDSTVIREEGIDELAKICGVEDAVSEMTRRAMGGAVPFKAALTERLALIQPSREQVQRLIAEQPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNIPATNVFANRLKFYFNGEYAGFDETQPTAESGGKGKVIKLLKEKFHFKKIIMIGDGATDMEACPPADAFIGFGGNVIRQQVKDNAKWYITDFVELLGELEE
• Specificity: PSPH - phosphoserine phosphatase
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSPH
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Phosphoserine phosphatase Antibody (3A5) - Azide and BSA Free

• L-3-phosphoserine phosphatase
• O-phosphoserine phosphohydrolase
• phosphoserine phosphatase
• PSPase
• PSPEC 3.1.3.3

Background

The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSPH
• Entrez:
  • 5723(Human)
• OMIM:
  • 172480(Human)
• Uniprot:
  • AAH63614(Human)