PHF7 Antibody (OTI1G9) - Azide and BSA Free

Western Blot: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHF7.

Immunocytochemistry/Immunofluorescence: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PHF7.

Flow Cytometry: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by PHF7 antibody.

Flow Cytometry: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371] - Analysis of Hela cells, using PHF7 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: PHF7 Antibody (OTI1G9) - Azide and BSA Free [NBP2-73371] - Analysis of Jurkat cells, using PHF7 antibody,(Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PHF7 Antibody (OTI1G9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PHF7 Antibody (OTI1G9) - Azide and BSA Free (NBP2-45881) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 155-381 of human PHF7(NP_057567) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PHF7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 43.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PHF7 Antibody (OTI1G9) - Azide and BSA Free

• DKFZp434L1850
• HSPC045
• HSPC226
• MGC26088
• NYD-SP6
• PHD finger protein 7
• Testis development protein NYD-SP6

Background

Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, this gene is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHF7