PGM3 Antibody (1E2-1B12) - Azide and BSA Free

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12.Lane 1: PGM3 transfected lysate(59.9 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 2 ug/ml.

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi ( Cat # H00005238-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody (M01), clone 1E2-1B12 (Cat # H00005238-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - PGM3 monoclonal antibody (M01), clone 1E2-1B12 Analysis of PGM3 expression in HeLa.

Immunoprecipitation: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 transfected lysate using anti-PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Detection limit for recombinant GST tagged PGM3 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP, KD
• Clone: 1E2-1B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PGM3 Antibody (1E2-1B12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PGM3 (AAH01258, 1 a.a. ~ 542 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHLDHVMFRMGLLAVLRSKQTKSTIGVMVTASHNPEEDNGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRVLIDISEKEAVNLQQDAFVVIGRDTRPSSEKLSQSVIDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRYGKATIEGYYQKLSKAFVELTKQASCSGDEYRSLKVDCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKLNHLCGADFVKSHQKPPQGMEIKSNERCCSFDGDADRIVYYYHDADGHFHLIDGDKIATLISSFLKELLVEIGESLNIGVVQTAYANGSSTRYLEEVMKVPVYCTKTGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEMKIKQSAEQLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVISTTNAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAGGIGERPQPGF
• Specificity: PGM3 - phosphoglucomutase 3
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGM3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PGM3 Antibody (1E2-1B12) - Azide and BSA Free

• AGM1PAGMAcetylglucosamine phosphomutase
• DKFZP434B187
• EC 5.4.2
• EC 5.4.2.3
• FLJ11614
• FLJ13623
• N-acetylglucosamine-phosphate mutase 1
• N-acetylglucosamine-phosphate mutase
• PGM 3
• phosphoacetylglucosamine mutase
• phosphoglucomutase 3
• Phosphoglucomutase-3

Background

Interconverts GlcNAc-6-P and GlcNAc-1-P

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGM3 Antibody (H00005238-M01)(1)

Publication using H00005238-M01

• Francesca R, Yang G, Roberta P et al. Hexosamine pathway inhibition overcomes pancreatic cancer resistance to gemcitabine through unfolded protein response and EGFR-Akt pathway modulation. Oncogene. 2020-03-31 [PMID: 32235891]

Bioinformatics

• Gene Symbol: PGM3
• Entrez:
  • 5238(Human)
• OMIM:
  • 172100(Human)
• Uniprot:
  • AAH01258(Human)