PGLYRP1/PGRP-S Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PGLYRP1/PGRP-S Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PGLYRP1/PGRP-S
  Cys17-Glu182
  Accession # O88593
• Specificity: Detects mouse PGRP‑S in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human PGRP-S is observed and less than 5% cross-reactivity with recombinant mouse PGRP-L is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Pglyrp1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PGLYRP1/PGRP-S Antibody

• peptidoglycan recognition protein 1
• Peptidoglycan recognition protein short
• PGLYRP
• PGLYRP1
• PGRPpeptidoglycan recognition protein
• PGRPS
• PGRP-S
• PGRP-SMGC126894
• PGRPSMGC126896
• TAG7
• TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein)
• TNFSF3L

Background

The mouse PGRP family comprises four peptidoglycan recognition proteins that may function as innate immunity pattern recognition molecules (1, 2). Termed PGRP‑L, PGRP-I alpha , PGRP-I beta and PGRP-S, they are all products of separate genes, and all are named for the relative length of their translated product (3). PGRP-L (for long) is 530 amino acids (aa) in length, while PGRP-I alpha and I beta are intermediate (I) in length at 347 aa and 374 aa, respectively, and PGRP-S is shortest at 182 aa in length (3, 4). All mouse PGRPs bind peptidoglycan and Gram-positive bacteria, and all have at least three C‑terminal PGRP domains at variable sites that are highly conserved from insects to mammals (3). Mouse PGRP-S, the first described member of the family, is an 18 kDa secreted protein associated with neutrophils (4, 5). The mature molecule is 166 aa in length and presumably contains three variably-sized peptide-carbohydrate recognition sequences. Mouse PGRP-S is 86%, 69% and 72% aa identical to rat, bovine and human mature PGRP-S, respectively. Studies with PGRP-S deficient mice indicate that knock-out mice have increased susceptibility to infections with low (but not high) pathogenicity bacteria. Neutrophils from knock-out mice exhibit normal phagocytosis of bacteria but are defective in intracellular killing and digestion of nonpathogenic bacteria (5). The three longer PGRP members are all membrane‑bound molecules that contain two membrane‑spanning segments. Both the N- and C‑termini are depicted as being extracellular with a joining cytoplasmic domain. All three transmembrane forms show at least one PGRP domain on the C‑terminal extracellular region; other PGRP domains are variably distributed over their two extracellular and one cytoplasmic region (3).

1. Girardin, S.E. and D.J. Philpott (2004) Eur. J. Immunol. 34:1777.
2. Steiner, H. (2004) Immunol. Rev. 198:83.
3. Kiselev, S.L. et al. (1998) J. Biol. Chem. 273:18633.
4. Kang, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10078.
5. Dziarski, R. et al. (2003) Blood 102:689.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGLYRP1/PGRP-S Antibody (AF2696)(2)

Publications using AF2696

• Chen, S;Putnik, R;Li, X;Diwaker, A;Vasconcelos, M;Liu, S;Gondi, S;Zhou, J;Guo, L;Xu, L;Temme, S;Bersch, K;Hyland, S;Yin, J;Burstein, E;Bahnson, BJ;Gildersleeve, JC;Grimes, CL;Reinecker, HC; PGLYRP1-mediated intracellular peptidoglycan detection promotes intestinal mucosal protection Nature communications 2025-02-21 [PMID: 39984444] (Immunohistochemistry, Western Blot, Human, Mouse)
• Ghosh A, Lee S, Dziarski R, Chakravarti S A novel antimicrobial peptidoglycan recognition protein in the cornea. Invest. Ophthalmol. Vis. Sci., 2009-04-22;50(9):4185-91. 2009-04-22 [PMID: 19387073] (IHC-Fr, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Pglyrp1
• Uniprot:
  • O88593()