PGK1 Antibody (2H4) - Azide and BSA Free

Western Blot: PGK1 Antibody (2H4) [H00005230-M02] - PGK1 monoclonal antibody (M02), clone 2H4. Analysis of PGK1 expression in HepG2.

Sandwich ELISA: PGK1 Antibody (2H4) [H00005230-M02] - Detection limit for recombinant GST tagged PGK1 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PGK1 Antibody (2H4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PGK1 (NP_000282, 321 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI
• Specificity: PGK1 - phosphoglycerate kinase 1 (2H4)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGK1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PGK1 Antibody (2H4) - Azide and BSA Free

• Cell migration-inducing gene 10 protein
• EC 2.7.2.3
• MGC142128
• MGC8947
• MIG10
• PGK1
• PGKA
• PGKAMGC117307
• phosphoglycerate kinase 1
• Primer recognition protein 2
• PRP 2
• PRP2

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. A pseudogene of this gene has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGK1
• Entrez:
  • 5230(Human)
• Uniprot:
  • NP_000282(Human)