PGAM1 Antibody (JE40-67)

Western Blot: PGAM1 Antibody (JE40-67) [NBP2-76862] - Western blot analysis of PGAM1 on different lysates using anti-PGAM1 antibody at 1/2,000 dilution.Positive control:Lane 1: A431 Lane 2: A549Lane 3: Rat brain Lane 4: Mouse brain

Immunohistochemistry: PGAM1 Antibody (JE40-67) [NBP2-76862] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PGAM1 antibody. Counter stained with hematoxylin.

Flow Cytometry: PGAM1 Antibody (JE40-67) [NBP2-76862] - Flow cytometric analysis of Jurkat cells with PGAM1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: PGAM1 Antibody (JE40-67) [NBP2-76862] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PGAM1 antibody. Counter stained with hematoxylin.

Product Discontinued

PGAM1 Antibody (JE40-67) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human PGAM1 aa 1-103 / 254. (SwissProt: P18669 Human; SwissProt: Q9DBJ1 Mouse; SwissProt: P25113 Rat)
• Localization: Cytosol. Extracellular region or secreted. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PGAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PGAM1 Antibody (JE40-67)

• BPG-dependent PGAM 1
• EC 3.1.3.13
• EC 5.4.2.1
• EC 5.4.2.4
• PGAMA
• PGAM-Bphosphoglycerate mutase 1
• phosphoglycerate mutase 1 (brain)
• phosphoglycerate mutase A, nonmuscle form
• Phosphoglycerate mutase isozyme B

Background

PGAM is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM in mammals, a muscle and brain isoform. PGAM has also been linked to non-spherocytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGAM1