![Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25598_rabbit-pfkfb4-mab-hl2204-azide-and-bsa-free-21202493912.jpg "Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | HL2204 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit PFKFB4 Antibody (HL2204) - Azide and BSA Free (NBP3-25598) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human PFKFB4. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PFKFB4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PFKFB4 |
![Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25598_rabbit-pfkfb4-mab-hl2204-azide-and-bsa-free-2120249374920.jpg "Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. PFKFB4 stained by PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25598_rabbit-pfkfb4-mab-hl2204-azide-and-bsa-free-2120249342796.jpg "Immunohistochemistry-Paraffin: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. PFKFB4 stained by PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25598_rabbit-pfkfb4-mab-hl2204-azide-and-bsa-free-212024941469.jpg "Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25598_rabbit-pfkfb4-mab-hl2204-azide-and-bsa-free-2120249425412.jpg "Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25598_rabbit-pfkfb4-mab-hl2204-azide-and-bsa-free-2120249362736.jpg "Western Blot: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. PFKFB4 stained by PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25598_rabbit-pfkfb4-mab-hl2204-azide-and-bsa-free-2120249371573.jpg "Immunohistochemistry-Paraffin: PFKFB4 Antibody (HL2204) - Azide and BSA Free [NBP3-25598] - PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. PFKFB4 stained by PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![PFKFB4 antibody [HL2204] detects PFKFB4 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.Green: PFKFB4 stained by PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25598_rabbit-pfkfb4-mab-hl2204-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518252020.jpg "PFKFB4 antibody [HL2204] detects PFKFB4 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.Green: PFKFB4 stained by PFKFB4 antibody [HL2204] (NBP3-25598) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")