Recombinant Human Peroxiredoxin 2 Protein

SDS-Page: Recombinant Human Peroxiredoxin 2 Protein [NBC1-25855] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human Peroxiredoxin 2 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-198 of Human Peroxiredoxin 2
  
  

  Source: E.coli

  Amino Acid Sequence: MASGNARIGK PAPDFKATAV VDGAFKEVKL SDYKGKYVVL FFYPLDFTFV CPTEIIAFSN RAEDFRKLGC EVLGVSVDSQ FTHLAWINTP RKEGGLGPLN IPLLADVTRR LSEDYGVLKT DEGIAYRGLF IIDGKGVLRQ ITVNDLPVGR SVDEALRLVQ AFQYTDEHGE VCPAGWKPGS DTIKPNVDDS KEYFSKHN

• Details of Functionality: Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PRDX2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 21.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Peroxiredoxin 2 Protein

• EC 1.11.1
• MGC4104
• Natural killer cell-enhancing factor B
• natural killer-enhancing factor B
• NKEF-B
• NKEFBNKEF-B
• Peroxiredoxin 2
• peroxiredoxin-2
• PRDX2
• PRPTDPX1
• PRX2
• PRXII
• TDPX1
• thiol-specific antioxidant 1
• Thiol-specific antioxidant protein
• Thioredoxin peroxidase 1
• Thioredoxin-dependent peroxide reductase 1
• torin
• TPX1
• TSA
• TSAEC 1.11.1.15

Background

Peroxiredoxin 2, also known as PRDX2, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Peroxiredoxin 2 may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. If Peroxiredoxin 2 protection is inadequate against peroxidases, the resulting protein and DNA damage may result in neurological disease such as Alzheimer's or DNA damage leading to cancer. Recombinant human Peroxiredoxin 2 protein was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Peroxiredoxin 2 Recombinant Protein (NBC1-25855)(1)

Publication using NBC1-25855

• Beyrath J, Pellegrini M, Renkema H et al. KH176 Safeguards Mitochondrial Diseased Cells from Redox Stress-Induced Cell Death by Interacting with the Thioredoxin System/Peroxiredoxin Enzyme Machinery Sci Rep 2018-04-26 [PMID: 29700325] (BA, Human)

Bioinformatics

• Gene Symbol: PRDX2