Peroxiredoxin 2 Antibody

Western Blot: Peroxiredoxin 2 Antibody [NB100-40806] - Analysis of Peroxiredoxin 2 using this antibody at 1 ug/ml in Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Peroxiredoxin 2 Antibody Summary

• Immunogen: Peptide with sequence C-NVDDSKEYFSKHN corresponding to C-Terminus according to NP_005800.3, NP_859427.1.
• Specificity: This antibody is expected to recognise isoforms a and b only (NP_005800.3 and NP_859427.1 respectively).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRDX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3-1 ug/ml
• Application Notes: Western blot: Approx 20-22kDa band observed in Human Brain lysates (calculated MW of 21.9kDa according to NP_005800.3). Primary incubation was 1 hour
• Control:

  Peroxiredoxin 2 Overexpression Lysate

Reactivity Notes

Expected from sequence similarity: Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Peroxiredoxin 2 Antibody

• EC 1.11.1
• MGC4104
• Natural killer cell-enhancing factor B
• natural killer-enhancing factor B
• NKEF-B
• NKEFBNKEF-B
• Peroxiredoxin 2
• peroxiredoxin-2
• PRDX2
• PRPTDPX1
• PRX2
• PRXII
• TDPX1
• thiol-specific antioxidant 1
• Thiol-specific antioxidant protein
• Thioredoxin peroxidase 1
• Thioredoxin-dependent peroxide reductase 1
• torin
• TPX1
• TSA
• TSAEC 1.11.1.15

Background

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms. Transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Peroxiredoxin 2 Antibody (NB100-40806)(1)

Publication using NB100-40806

• Kato S, Kato M, Abe Y et al. Redox system expression in the motor neurons in amyotrophic lateral sclerosis (ALS): immunohistochemical studies on sporadic ALS, superoxide dismutase 1 (SOD1)-mutated familial ALS, and SOD1-mutated ALS animal models. Acta Neuropathol 2005-06-28 [PMID: 15983830]

Bioinformatics

• Gene Symbol: PRDX2
• Entrez:
  • 7001(Human)
  • 21672(Mouse)
• Uniprot:
  • NP_005800.3(Human)