Perilipin Antibody

Western Blot: Perilipin Antibody [NB100-60554] - (1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Western Blot: Perilipin Antibody [NB100-60554] - (2ug/ml) staining of Human Breast (A) and (1ug/ml) Breast cancer (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Product Discontinued

Perilipin Antibody Summary

• Immunogen: Peptide with sequence C-EPILGRAQYSQLRKK corresponding to C-Terminus according to NP_002657.2.
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLIN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: ICC/IF, IHC-Frozen reactvity reported in PMID: 21420243, IHC-P reactivity reported in PMID: 29867212.
  WB: Approx 55kDa band observed in Human Breast and Breast cancer lysates, and in lysates of cell line Jurkat (calculated MW of 56.0kDa according to NP_002657.3).
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine. Mouse reactivity reported in scientific literature (PMID: 21407813). Porcine reactivity reported in scientific literature (PMID: 21420243).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Perilipin Antibody

• PERI
• perilipin 1
• Perilipin
• perilipin-1
• PLIN
• PLIN1
• PLINLipid droplet-associated protein

Background

The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Perilipin Antibody (NB100-60554)(17)

Publications using NB100-60554

• Zhao S, Lin Q, Xiong W et al. Hyperleptinemia contributes to antipsychotic drug-associated obesity and metabolic disorders Science translational medicine 2023-11-22 [PMID: 37992151] (IHC, Mouse)
• Liu X, Gu Y, Kumar S et al. Oxylipin-PPAR?-initiated adipocyte senescence propagates secondary senescence in the bone marrow Cell metabolism 2023-04-04 [PMID: 37019080]
• Kwon J, Yeh Y, Kawarasaki S et al. Mevalonate biosynthesis pathway regulates the development and survival of brown adipocytes iScience 2023-03-01 [PMID: 36895651] (ICC/IF, Mouse)
• Ren L, Du W, Song D et al. Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice iScience 2023-01-20 [PMID: 36594018]
• Jing X, Wu J, Dong C et al. COVID-19 instigates adipose browning and atrophy through VEGF in small mammals Nature metabolism 2022-12-01 [PMID: 36482111] (IHC-P, Mouse, Hamster)
• Wang Y, Peng Y, Yang Y et al. The effect of the PLIN1 gene on the metabolism and mitochondria of porcine skeletal muscle satellite cells Genes & genomics 2022-04-19 [PMID: 35438463]
• Li N, Zhang Z, Zhao S, et al. Adiponectin Preserves Metabolic Fitness During Aging Elife 2021-04-27 [PMID: 33904399]
• Wang Z, Ning T, Song A et al. Chronic cold exposure enhances glucose oxidation in brown adipose tissue EMBO Rep 2020-10-12 [PMID: 33043581] (IHC-P, Mouse)
• Zhao S, Li N, Zhu Y et al. Partial leptin deficiency confers resistance to diet-induced obesity in mice Mol Metab 2020-04-11 [PMID: 32289482] (IHC-P, IF/IHC, Mouse)
• Sun C, Sakashita H, Kim J et al. Mosaic Mutant Analysis Identifies PDGFR alpha/PDGFR beta as Negative Regulators of Adipogenesis Cell Stem Cell 2020-03-25 [PMID: 32229310] (ICC/IF, Mouse)
• Nakazeki F, Nishiga M, Horie T et al. Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo Sci Rep 2018-06-04 [PMID: 29867212] (IHC-P, Mouse)
• Wang QA, Zhang F, Jiang L et al. PPARgamma and its Role in Adipocyte Homeostasis and Thiazolidinedione-Mediated Insulin Sensitization. Mol. Cell. Biol. 2018-02-26 [PMID: 29483301] (IHC-P, Mouse)
• Sun C, Berry WL, Olson LE. PDGFRa controls the balance of stromal and adipogenic cells during adipose tissue organogenesis. Development 2017-01-01 [PMID: 28049691] (Mouse)
• Wang QA, Tao C, Jiang L et al. Distinct regulatory mechanisms governing embryonic versus adult adipocyte maturation. Nat. Cell Biol. 2015-09-01 [PMID: 26280538] (IHC-P, Mouse)
• Gandolfi G, Mazzoni M, Zambonelli P, Lalatta-Costerbosa G, Tronca A, Russo V, Davoli R. Perilipin 1 and perilipin 2 protein localization and gene expression study in skeletal muscles of European cross-breed pigs with different intramuscular fat contents. Meat Sci;88(4):631-7. 2011-08-01 [PMID: 21420243] (IHC-Fr, ICC/IF, Porcine)
• Rigamonti A, BrennK, Lau F, Cowan CA. Rapid cellular turnover in adipose tissue. PLoS ONE. 2011-01-01 [PMID: 21407813]
• Wolins NE, Brasaemle DL, Bickel PE. A proposed model of fat packaging by exchangeable lipid droplet proteins. FEBS Lett 2006-10-09 [PMID: 16962104]

Bioinformatics

• Gene Symbol: PLIN1
• Entrez:
  • 5346(Human)
  • 103968(Mouse)
  • 25629(Rat)
• Uniprot:
  • O60240(Human)