Pepsinogen I Antibody (8003 (99/12)) - BSA Free

Immunohistochemistry-Paraffin: Pepsinogen I Antibody (8003 (99/12)) [NB100-62239] - Differentiation of pyloric-gland type to fundic glands. (A) HE-stained image, showing proliferation of atypical glands associated with mildly enlarged nuclei. (B) Differentiation into fundic glands (pepsinogen-1). Tumor glands are positive for pepsinogen-1. (C) Differentiation into fundic glands (H,K-ATPase). Similar to pepsinogen-1, H,K-ATPase-positive cells can be seen in tumor glands. From: Mitsuishi T, Hamatani S, Hirooka S, Fukasawa N, Aizawa D, Hara Y, et al. (2017)

• Reactivity: Hu
• Applications: ELISA, IHC, IHC-P, RIA
• Clone: 8003 (99/12)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Pepsinogen I Antibody (8003 (99/12)) - BSA Free Summary

• Immunogen: Purified human pepsinogen 1.
• Localization: Secreted
• Specificity: NB100-62239 recognises human Pepsinogen I, a zymogen or proenzyme secreted by Chief cells in the stomach. It is cleaved to form pepsin both in an autocatalytic fashion and by pepsin itself. In humans there are two related forms of pepsin, Pepsinogen I (also known as pepsinogen A), and Pepsinogen II (also known as Pepsinogen B or progastricsin).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGA3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Radioimmunoassay 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for Pepsinogen I Antibody (8003 (99/12)) - BSA Free

• pepsinogen 3, group I (pepsinogen A)

Background

Pepsinogen consists of a single polypeptide chain of 375 amino acids with an average molecular weight of 42 kDa. Pepsinogen I is synthesized by gastric chief cells and mucous neck cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pepsinogen I Antibody (NB100-62239)(1)

Publication using NB100-62239

• Yang G Microsatellite instability/mismatch repair deficiency and activation of the Wnt/ beta-catenin signaling pathway in gastric adenocarcinoma of the fundic gland: A case report Medicine 2022-08-26 [PMID: 36042639] (IHC-P, Human)
  
  Details:
  Dilution used for IHC 1:200

Bioinformatics

• Gene Symbol: PGA3