PEG10 Antibody (4C10A7)

Western Blot: PEG10 Antibody (4C10A7) [NBP1-28900] - Western blot analysis using PEG10 mouse mAb against HepG2 (1), SMMC-7721 (2) and A549 (3) cell lysate.

Immunohistochemistry-Paraffin: PEG10 Antibody (4C10A7) [NBP1-28900] - Immunohistochemical analysis of paraffin-embedded human hepatocarcinoma (A), breast carcinoma (B) and lung cancer tissues (C), showing cytoplasmic localization with DAB staining using PEG10 mouse mAb.

Immunohistochemistry-Paraffin: PEG10 Antibody (4C10A7) [NBP1-28900] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using PEG10 mouse mAb

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clone: 4C10A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PEG10 Antibody (4C10A7) Summary

• Immunogen: Purified recombinant fragment of human PEG10 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PEG10
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31400114).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for PEG10 Antibody (4C10A7)

• EDR
• embryonal carcinoma differentiation regulated
• Embryonal carcinoma differentiation-regulated protein
• HB-1
• KIAA1051EDR
• Mammalian retrotransposon-derived protein 2
• Mar2
• Mart2
• MEF3 Like 1
• MEF3L
• MEF3L1
• Myelin expression factor 3-like protein 1
• paternally expressed 10
• Paternally expressed gene 10 protein
• PEG10
• retrotransposon gag domain containing 3
• Retrotransposon gag domain-containing protein 3
• Retrotransposon-derived gag-like polyprotein
• retrotransposon-derived protein PEG10
• RGAG3
• RGAG3MEF3-like protein 1
• RTL2
• SIRH1
• Ty3/Gypsy-like protein

Background

This gene includes two overlapping reading frames of the same transcript encoding distinct isoforms. The shorter isoform has a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons, and it functions in part by interacting with members of the TGF-beta receptor family. The longer isoform has the active-site DSG consensus sequence of the protease domain of pol proteins. The longer isoform is the result of -1 translational frame shifting that is also seen in some retroviruses. Expression of these two isoforms only comes from the paternal allele due to imprinting. Increased gene expression (as observed by an increase in mRNA levels) is associated with hepatocellular carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEG10 Antibody (NBP1-28900)(1)

Publication using NBP1-28900

• Uxa S, Bernhart SH, Mages CFS et al. DREAM and RB cooperate to induce gene repression and cell-cycle arrest in response to p53 activation Nucleic Acids Res. 2019-08-10 [PMID: 31400114] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: PEG10