Pea3 Antibody (1A2G3)

Western Blot: Pea3 Antibody (1A2G3) [NBP1-28856] - Western blot analysis using ETV4 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY ETV4 cDNA (2).

Western Blot: Pea3 Antibody (1A2G3) [NBP1-28856] - analysis using anti-ETV4 mAb against K562 cell lysate (1).

Product Discontinued

Pea3 Antibody (1A2G3) Summary

• Immunogen: Purified recombinant fragment of human ETV4 (aa50-109) expressed in E. Coli.
• Specificity: Specific for ETV4.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ETV4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500-1:2000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Pea3 Antibody (1A2G3)

• Adenovirus E1A enhancer-binding protein
• E1A-FE1A enhancer binding protein
• E1AFETS translocation variant 4
• ets variant 4
• ets variant gene 4 (E1A enhancer binding protein, E1AF)
• ets variant gene 4 (E1A enhancer-binding protein, E1AF)
• EWS protein/E1A enhancer binding protein chimera
• PEA3
• PEAS3
• Polyomavirus enhancer activator 3 homolog
• polyomavirus enhancer activator-3
• Protein PEA3

Background

ETV4: ets variant gene 4 (E1A enhancer binding protein, E1AF). Several members of the Ets gene family are known to encode sequence specific DNA binding proteins. These include mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-alpha. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU.1, although PU.1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pea3 Antibody (NBP1-28856)(1)

Publication using NBP1-28856

• Wong D Functional Investigations of Capicua (CIC) and ATAXIN-1-Like (ATXN1L) in cancer. Thesis 2020-01-01 (WB, Human)

Bioinformatics

• Gene Symbol: ETV4