Recombinant Human PDXP His Protein

Western Blot: PDXP Protein [NBP1-45304] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human PDXP His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-296 of Human PDXP
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MARCERLRGA ALRDVLGRAQ GVLFDCDGVL WNGERAVPGA PELLERLARA GKAALFVSNN SRRARPELAL RFARLGFGGL RAEQLFSSAL CAARLLRQRL PGPPDAPGAV FVLGGEGLRA ELRAAGLRLA GDPSAGDGAA PRVRAVLVGY DEHFSFAKLR EACAHLRDPE CLLVATDRDP WHPLSDGSRT PGTGSLAAAV ETASGRQALV VGKPSPYMFE CITENFSIDP ARTLMVGDRL ETDILFGHRC GMTTVLTLTG VSRLEEAQAY LAAGQHDLVP HYYVESIADL TEGLED

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PDXP
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 33.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human PDXP His Protein

• chronophin
• CIN
• dJ37E16.5
• EC 3.1.3
• EC 3.1.3.74
• FLJ32703
• PLP phosphatase
• PLP
• PLPP
• pyridoxal (pyridoxine, vitamin B6) phosphatase
• pyridoxal phosphate phosphatase

Background

Pyridoxal phosphate phosphatase, also known as PDXP is the enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. PDXP catalyzes the dephosphorylation of pyridoxal 5'-phosphate and exhibits a high level of expression various parts of the central nervous system, especially the brain. PDXP activity is catalyzed by haloacid dehalogenase (HAD), and it is the cofactor of both aromatic L-amino acid decarboxylase and glutamate decarboxylase. Recombinant human PDXP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PDXP