PDLIM4 Antibody

Images

 
Western Blot: PDLIM4 Antibody [NB100-1382] - Staining of A431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PDLIM4 Antibody [NB100-1382] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PDLIM4 Antibody Summary

Immunogen
Peptide with sequence C-DVVAVYPNAKVELV corresponding to C-Terminus according to NP_003678.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDLIM4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.5 ug/ml
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 37 kDa band observed in A431 lysates (predicted MW of approx. 35 kDa band according to NP_003678). IHC: Paraffin embedded Human Breast.
Positive Control
A-431 Lysate (NB820-59461)
Rab13 Lysate (NBL1-15040)
Publications
Read Publication using NB100-1382.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDLIM4 Antibody

  • LIM domain protein
  • LIM protein RIL
  • PDZ and LIM domain 4
  • PDZ and LIM domain protein 4
  • Reversion-induced LIM protein
  • RILenigma homolog

Background

RIL was originally identified as a gene downregulated in H-ras-transformed cells. RIL was mostly expressed in nonmuscle epithelial cells with a pattern distinct from that of CLP-36. RIL protein was found to localize to actin stress fibers in nonmuscle cells similarly to CLP-36. However, RIL expression led to partially abnormal actin filaments showing thick irregular stress fibers not seen with CLP-36. The RIL PDZ-LIM protein is a regulator of actin stress fiber turnover.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDLIM4 Antibody (NB100-1382)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDLIM4
Entrez
Uniprot