PDLIM4 Antibody

Western Blot: PDLIM4 Antibody [NB100-1382] - (1ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence

Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NB100-1382] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NB100-1382] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PDLIM4 Antibody Summary

• Immunogen: Peptide with sequence C-DVVAVYPNAKVELV corresponding to C-Terminus according to NP_003678.2.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDLIM4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1.0 -3.0 ug/ml
• Application Notes: Western blot: Approx 37kDa band observed in lysates of cell line A431 (calculated MW of 35.4kDa according to NP_003678.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
• Control:

  A-431 Whole Cell Lysate
  Rab13 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDLIM4 Antibody

• LIM domain protein
• LIM protein RIL
• PDZ and LIM domain 4
• PDZ and LIM domain protein 4
• Reversion-induced LIM protein
• RILenigma homolog

Background

RIL was originally identified as a gene downregulated in H-ras-transformed cells. RIL was mostly expressed in nonmuscle epithelial cells with a pattern distinct from that of CLP-36. RIL protein was found to localize to actin stress fibers in nonmuscle cells similarly to CLP-36. However, RIL expression led to partially abnormal actin filaments showing thick irregular stress fibers not seen with CLP-36. The RIL PDZ-LIM protein is a regulator of actin stress fiber turnover.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDLIM4 Antibody (NB100-1382)(3)

Publications using NB100-1382

• Monlau F, Sambusiti C, Barakat A. Comparison of Dry Versus Wet Milling to Improve Bioethanol or Methane Recovery from Solid Anaerobic Digestate Bioengineering 2019-09-06 [PMID: 31500163]
• Perumpail BJ, Li AA, Iqbal U et al. Potential Therapeutic Benefits of Herbs and Supplements in Patients with NAFLD Diseases 2018-09-10 [PMID: 30201879]
• Bashirova AA, Markelov ML, Shlykova TV et al. The human RIL gene: mapping to human chromosome 5q31.1, genomic organization and alternative transcripts. Gene 1998-04-14 [PMID: 9573374]

Bioinformatics

• Gene Symbol: PDLIM4
• Entrez:
  • 8572(Human)
  • 30794(Mouse)
  • 24915(Rat)
• Uniprot:
  • NP_003678(Human)