Rat PDK4 ELISA Kit (Colorimetric)

ELISA: Rat PDK4 ELISA Kit (Colorimetric) [NBP2-81233] - Samples were spiked with high concentrations of Rat PDK4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat PDK4 ELISA Kit (Colorimetric) [NBP2-81233] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Rat PDK4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat PDK4 in samples. No significant cross-reactivity or interference between Rat PDK4 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.08%
• Intra-Assay: CV% < 5.06%
• Spike Recovery: 86-103%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PDK4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat PDK4 ELISA Kit (Colorimetric)

• [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
• EC 2.7.11
• EC 2.7.11.2
• FLJ40832
• Pyruvate dehydrogenase kinase isoform 4
• pyruvate dehydrogenase kinase, isoenzyme 4
• pyruvate dehydrogenase kinase, isozyme 4
• pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial

Background

Pyruvate dehydrogenase kinase isoform 4 (PDK4) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

PDK4 antibodies are useful tools for glucose metabolism research and mitochondiral metabolism studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PDK4 ELISA Kit (NBP2-81233). (Showing 1 - 2 of 2 FAQ).

1. Which PDK4 antibody do you recommend for immunoblot? I'm working with human cell lines.
   • NBP1-07047 and NBP1-07049 are our best selling PDK4 antibodies that will meet your mentioned criteria i.e. Immunoblot/Western Blot in Human samples. However, all of our products are 100% guaranteed and you may find a complete list of PDK4 antibodies by searching PDK4 on our website and narrowing your results using the filters on the left-hand side of the screen.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PDK4