PDGFRL Antibody (1010639) [Unconjugated]

Recombinant Mouse PDGFRL (Catalog # 9739-PR) stimulates proliferation in the HCT-116 human colorectal carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse PDGFRL (10 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse PDGFRL Monoclonal Antibody (Catalog # MAB9739). The ND50 is typically 4-24 µg/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 1010639
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

PDGFRL Antibody (1010639) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell HEK293-derived mouse PDGFRL
  Gln22-Ser375
  Accession # Q6PE55
• Specificity: Detects mouse PDGFRL in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 4-24 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGFRL Antibody (1010639) [Unconjugated]

• PDGFRL
• PDGFR-Like Protein
• PDGRL
• platelet-derived growth factor receptor-like protein
• platelet-derived growth factor receptor-like
• platelet-derived growth factor-beta-like tumor suppressor
• PRLTS
• PRLTSPDGF receptor beta-like tumor suppressor

Background

Platelet-derived growth factor receptor-like protein (PDGFRL) is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, mouse PDGFRL is 90% similar to the human version and 95% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). The high frequency of loss of heterozygosity (LOH) near the gene of PDGFRL indicated in several studies that PDGFRL can be a tumor suppressor gene (TSG) in breast cancer (6-9), colorectal cancer, prostate cancer (10), non-small cell lung cancers (11) and hepatocellular carcinoma (12). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (13). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (14). Another study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation (15).

1. Guo, F-J. et al. (2010). World J Gastroenterol. 16:1465.
2. Andrae, J. et al. (2008) Genes Dev. 22:1276.
3. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
4. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
5. Matsui, T. et al. (1989) Science 243:800.
6. Seitz, S. et al. (2006). Genes Chromosomes Cancer 45:612.
7. Yaremko, M.L. et al. (1996). Genes, Chromosome Cancer 16:189.
8. Seitz, S. et al. (2000). Eur J Cancer. 36:1507.
9. Rennstam, K. et al. (2003). Cancer Res. 63:8861.
10. Komiya, A. et al. (1997). Jpn J Cancer Res. 88:389.
11. Lerebours, F. et al. (1999). Int J Cancer. 81:854.
12. Kahng, Y.S. et al. (2003) J Gastroenterol Hepatol. 18:430.
13. Hou, S. et al. (2013). Hum Mutat. 34:74.
14. Fujikado N. et al. (2006). Arthritis Res.& Ther. 8:R100.
15. Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q6PE55()