PDGFR alpha Antibody

Product Discontinued

PDGFR alpha Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 1020-1070 of Human PDGFR-alpha.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDGFRA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of PDGFR-alpha (S1057) pAb in extracts from HepG2 cells.
  Immunohistochemistry (IHC) analyzes of PDGFR-alpha (S1057) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDGFR alpha Antibody

• alpha-type platelet-derived growth factor receptor
• CD140 antigen-like family member A
• CD140a antigen
• CD140a
• EC 2.7.10
• EC 2.7.10.1
• MGC74795
• PDGF R alpha
• PDGFR alpha
• PDGFR2
• PDGFRA
• PDGFRA/BCR fusion
• PDGF-R-alpha
• platelet-derived growth factor receptor, alpha polypeptide
• rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
• RHEPDGFRA

Background

Platelet-derived growth factor receptors exhibit tyrosine-protein kinase activity and have been implicated in the control of cell proliferation, survival and migration. PDGF receptors, PDGFR-alpha and PDGFR-beta, have 5 extracellular immunoglobulin-like domains and an intracellular tyrosine kinase domain. Upon binding a PDGF, the receptors form homo-and heterodimers. Dimerization of the receptors results in phosphorylation in the complex. More than 10 different SH2-domain-containing molecules have been shown to bind to different autophosphorylation sites in the PDGF-alpha and beta receptors. PDGF alpha receptors are expressed in oligodendrocyte progenitor cells and PDGF beta receptors are expressed on neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFR alpha Antibody (NBP1-19423)(2)

Publications using NBP1-19423

• Hakim GD, Soyturk M, Unlu M, Ataca P. Mucosal healing effect of nilotinib in indomethacin-induced enterocolitis: A rat model. World Journal of Gastroenterol. 2015-11-28 [PMID: 26640333] (IHC-P, Rat)
• Ataca P, Soyturk M, Karaman M et al. Nilotinib-mediated mucosal healing in a rat model of colitis. World J Gastroenterol. 2013-10-07 [PMID: 24115822] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: PDGFRA
• Entrez:
  • 5156(Human)
  • 18595(Mouse)
  • 25267(Rat)
• Uniprot:
  • P16234()