PDGFA Antibody (MM0013-6D35)

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Immunohistochemistry-Paraffin: PDGFA Antibody (MM0013-6D35) [NB110-60979] - Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using NB110-60979 (Proteinase K pretreatment ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
MM0013-6D35
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

PDGFA Antibody (MM0013-6D35) Summary

Immunogen
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human PDGF-AA recombinant protein.
Specificity
PDGF AA (MM0013-6D35)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PDGFA
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry 1:50-200
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
In western blots this antibody shown no cross-reactivity with E-Cadherin, and P-Cadherin. In Western blot

Reactivity Notes

This antibody is reactive against human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for PDGFA Antibody (MM0013-6D35)

  • PDGF-1
  • PDGF1PDGF A-chain
  • PDGF-A
  • Platelet-derived growth factor A chain
  • platelet-derived growth factor alpha chain
  • platelet-derived growth factor alpha isoform 2 preproprotein
  • platelet-derived growth factor alpha polypeptidePDGF subunit A
  • platelet-derived growth factor subunit A

Background

PDGF AA is a member of the platelet derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGF AA can exist either as a homodimer or as a heterodimer with the platelet derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Blogs on PDGFA.

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Bioinformatics

Gene Symbol PDGFA
Entrez