PDGFA Antibody (MM0013-6D35) - Azide and BSA Free

Western Blot: PDGFA Antibody (MM0013-6D35) - Azide and BSA Free [NB110-60979] - Lysate of human placenta tissue with mouse anti-human PDGFA antibody.

Immunohistochemistry-Paraffin: PDGFA Antibody (MM0013-6D35) [NB110-60979] - Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using NB110-60979 (Proteinase K pretreatment is required).

• Reactivity: Hu
• Applications: WB, IHC
• Clone: MM0013-6D35
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PDGFA Antibody (MM0013-6D35) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PDGFA Antibody (MM0013-6D35) - Azide and BSA Free (NB110-60979) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant PDGFA
• Specificity: In western blots this antibody shown no cross-reactivity with E-Cadherin, and P-Cadherin. There is 25% crossreactivitywith rhPDGF-AB, and no cross-reactivity with rhPDGF-BB.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000-1:2000

Reactivity Notes

This antibody is reactive against human. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for PDGFA Antibody (MM0013-6D35) - Azide and BSA Free

• PDGF-1
• PDGF1PDGF A-chain
• PDGF-A
• Platelet-derived growth factor A chain
• platelet-derived growth factor alpha chain
• platelet-derived growth factor alpha isoform 2 preproprotein
• platelet-derived growth factor alpha polypeptidePDGF subunit A
• platelet-derived growth factor subunit A

Background

PDGF AA is a member of the platelet derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGF AA can exist either as a homodimer or as a heterodimer with the platelet derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDGFA
• Entrez:
  • 5154(Human)