Recombinant Human PDGF R beta Protein

Product Discontinued

Recombinant Human PDGF R beta Protein Summary

• Description: Recombinant protein for Human PDGF R beta
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NM_002609)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: PDGFRB
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 74C under standard DNA polymerase assay conditions.
  Specific activity: The specific activity of PDGFRb was determined to be 20 nmol /min/mg as per activity assay protocol.
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PDGF R beta Protein

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

PDGFRb (platelet-derived growth factor receptor b) is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity. PDGFRb deficient mice are hemorrhagic, severely anemic and exhibit a defect in kidney glomeruli function (1). However, absence of PDGFRb has no impact on major blood vessels and the heart. PDGFRb expression and activity is elevated in several cancers and inhibition of PDGFRb activity blocks progression of renal carcinoma in an animal model (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PDGFRB