PDGF R beta Antibody (MM0005-5C37)

Product Discontinued

PDGF R beta Antibody (MM0005-5C37) Summary

• Immunogen: Recombinant hPGFDR beta N-terminal
• Specificity: This antibody detects human PDGFRb in western blots. No cross-reactivity is shown with hPDGFRa
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFRB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 12-5 ul/10 6 cells
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50 -500
  • Immunohistochemistry-Paraffin 1:50-500
  • Western Blot 1:400-1:1000
• Application Notes: This antibody is Cytof ready. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30125282).
• Control:

  PDGF R beta Overexpression Lysate

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 30125282).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for PDGF R beta Antibody (MM0005-5C37)

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

Platelet-derived growth factors (PDGFs) exhibit tyrosine-protein kinase activity and have been implicated in the control of cell proliferation, survival and migration. PDGF receptors, PDGFR-alpha and PDGFR-beta, have 5 extracellular immunoglobulin-like domains and an intracellular tyrosine kinase domain. Upon binding a PDGF, the receptors form homo-and heterodimers. Dimerization of the receptors results in phosphorylation in the complex. More than 10 different SH2-domain-containing molecules have been shown to bind to different autophosphorylation sites in the PDGF-alpha and beta receptors. PDGF alpha receptors are expressed in oligodendrocyte progenitor cells and PDGF beta receptors are expressed on neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGF R beta Antibody (NB110-60970)(2)

Publications using NB110-60970

• Szubert, S;Moszynski, R;Szpurek, D;Romaniuk, B;Sajdak, S;Nowicki, M;Michalak, S; The expression of Platelet-Derived Growth Factor Receptors (PDGFRs) and their correlation with overall survival of patients with ovarian cancer Ginekol. Pol. 2019-04-12 [PMID: 30977516] (IF/IHC, Human)
• Mao Y, Liu X, Song Y et al. VEGF-A/VEGFR-2 and FGF-2/FGFR-1 but not PDGF-BB/PDGFR-b play important roles in promoting immature and inflammatory intraplaque angiogenesis PLoS ONE 2018-08-20 [PMID: 30125282] (ICC/IF, IF/IHC, WB, Rabbit)

Bioinformatics

• Gene Symbol: PDGFRB
• Entrez:
  • 5159(Human)
• Uniprot:
  • P09619()