PDE6D Antibody (5C7) - Azide and BSA Free

Western Blot: PDE6D Antibody (5C7) [H00005147-M06] - Analysis of PDE6D expression in HeLa (Cat # L013V1).

Immunocytochemistry/Immunofluorescence: PDE6D Antibody (5C7) [H00005147-M06] - Analysis of monoclonal antibody to PDE6D on HeLa cell. Antibody concentration 20 ug/ml

ELISA: PDE6D Antibody (5C7) [H00005147-M06] - Detection limit for recombinant GST tagged PDE6D is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PDE6D Antibody (5C7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PDE6D (AAH07831, 1 a.a. ~ 150 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV
• Localization: Cytoplasmic
• Specificity: PDE6D - phosphodiesterase 6D, cGMP-specific, rod, delta
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDE6D
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PDE6D Antibody (5C7) - Azide and BSA Free

• GMP-PDE delta
• PDE6D
• PDED
• phosphodiesterase 6D, cGMP-specific, rod, delta
• Protein p17
• retinal rod rhodopsin-sensitive cGMP 3'-5'-cyclic phosphodiesterase subunitdelta

Background

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alpha/beta) that is regulated by two inhibitory y subunits. As PDE6D does not modify the catalytic properties of PDEalpha/beta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE6D
• Entrez:
  • 5147(Human)
• OMIM:
  • 602676(Human)
• Uniprot:
  • AAH07831(Human)