PDE1B Antibody (OTI6E4)

Images

 
Western Blot: PDE1B Antibody (6E4) [NBP2-01063] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: PDE1B Antibody (6E4) [NBP2-01063] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B.
Immunohistochemistry-Paraffin: PDE1B Antibody (6E4) [NBP2-01063] - Staining of paraffin-embedded Human Kidney tissue using anti-PDE1B mouse monoclonal antibody.
Flow Cytometry: PDE1B Antibody (6E4) [NBP2-01063] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE1B antibody, and then analyzed ...read more
Flow Cytometry: PDE1B Antibody (6E4) [NBP2-01063] - Analysis of Jurkat cells, using anti-PDE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI6E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PDE1B Antibody (OTI6E4) Summary

Immunogen
Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PDE1B
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for PDE1B Antibody (OTI6E4)

  • 63 kDa Cam-PDE
  • calcium/calmodulin-dependent 3'-5'-cyclic nucleotide phosphodiesterase 1B
  • Cam-PDE 1B
  • EC 3.1.4
  • EC 3.1.4.17
  • PDE1B1calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase
  • PDES1Bcalmodulin-stimulated phosphodiesterase PDE1B1
  • phosphodiesterase 1B, calmodulin-dependent
  • phosphodiesterase IB, calmodulin-dependent
  • Phosphodiesterase-1B
  • presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase familyknown as PDE1B

Background

Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDE1B Antibody (NBP2-01063) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73278AF350
Alexa Fluor 405 NBP2-73278AF405
Alexa Fluor 488 NBP2-73278AF488
Alexa Fluor 532 NBP2-73278AF532
Alexa Fluor 594 NBP2-73278AF594
Alexa Fluor 647 NBP2-73278AF647
Alexa Fluor 700 NBP2-73278AF700
Alexa Fluor 750 NBP2-73278AF750
Allophycocyanin NBP2-73278APC
Biotin NBP2-73278B
DyLight 350 NBP2-73278UV
DyLight 405 NBP2-73278V
DyLight 488 NBP2-73278G
DyLight 550 NBP2-73278R
DyLight 594 NBP2-73278DL594
DyLight 650 NBP2-73278C
DyLight 680 NBP2-73278FR
DyLight 755 NBP2-73278IR
FITC NBP2-73278F
HRP NBP2-73278H
Janelia Fluor 549 NBP2-73278JF549
Janelia Fluor 646 NBP2-73278JF646
PE NBP2-73278PE
PerCP NBP2-73278PCP

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Bioinformatics

Gene Symbol PDE1B
Entrez
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