PDE1B Antibody (OTI6E4)

Western Blot: PDE1B Antibody (6E4) [NBP2-01063] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B.

Immunocytochemistry/Immunofluorescence: PDE1B Antibody (6E4) [NBP2-01063] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B.

Immunohistochemistry-Paraffin: PDE1B Antibody (6E4) [NBP2-01063] - Staining of paraffin-embedded Human Kidney tissue using anti-PDE1B mouse monoclonal antibody.

Flow Cytometry: PDE1B Antibody (6E4) [NBP2-01063] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE1B antibody, and then analyzed by flow cytometry.

Flow Cytometry: PDE1B Antibody (6E4) [NBP2-01063] - Analysis of Jurkat cells, using anti-PDE1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI6E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PDE1B Antibody (OTI6E4) Summary

• Immunogen: Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDE1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 61.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE1B Antibody (OTI6E4)

• 63 kDa Cam-PDE
• calcium/calmodulin-dependent 3'-5'-cyclic nucleotide phosphodiesterase 1B
• Cam-PDE 1B
• EC 3.1.4
• EC 3.1.4.17
• PDE1B
• PDE1B1calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase
• PDES1B
• PDES1Bcalmodulin-stimulated phosphodiesterase PDE1B1
• phosphodiesterase 1B, calmodulin-dependent
• phosphodiesterase IB, calmodulin-dependent
• Phosphodiesterase-1B
• presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase familyknown as PDE1B

Background

Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDE1B
• Entrez:
  • 5153(Human)