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PDE1A Antibody (OTI7D5)

Images

 
Western Blot: PDE1A Antibody (7D5) [NBP2-46355] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1A .
Immunohistochemistry: PDE1A Antibody (7D5) [NBP2-46355] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PDE1A Antibody (7D5) [NBP2-46355] - Analysis of Adenocarcinoma of Human breast tissue using PDE1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C ...read more
Immunohistochemistry: PDE1A Antibody (7D5) [NBP2-46355] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PDE1A Antibody (7D5) [NBP2-46355] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PDE1A Antibody (7D5) [NBP2-46355] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PDE1A Antibody (7D5) [NBP2-46355] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI7D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PDE1A Antibody (OTI7D5) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 259-545 of human PDE1A (NP_005010) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PDE1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDE1A Antibody (OTI7D5)

  • 3'-5' cyclic nucleotide phosphodiesterase
  • 61 kDa Cam-PDE
  • calcium/calmodulin-dependent 3'-5'-cyclic nucleotide phosphodiesterase 1A
  • calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase
  • calmodulin-dependent phosphodiesterase
  • Cam-PDE 1A
  • EC 3.1.4
  • EC 3.1.4.17
  • HCAM1
  • hCam-1
  • HSPDE1A
  • MGC26303
  • PDE1A
  • phosphodiesterase 1A, calmodulin-dependent
  • phosphodiesterase-1A

Background

PDE1A, a variant of PDE1, is a Ca2+-calmodulin-stimulated phosphodieterase (PDE) preferentially hydrolyzing cGMP. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, B, and C). PDE1A and PDE1B have higher affinity for cGMP than for cAMP, whereas PDE1C has high affinity for both cAMP and cGMP. The PDE1A gene family includes various proteins that differ from each other at amino and carboxy termini of the protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PDE1A