DARPP-32 Antibody (375604) [Unconjugated]

Western blot shows lysates of mouse brain corpus striatum tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat DARPP-32 Monoclonal Antibody (Catalog # MAB4230) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DARPP-32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clone: 375604
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

DARPP-32 Antibody (375604) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human DARPP-32
  Ala51-Thr204
  Accession # Q9UD71.2
• Specificity: Detects mouse and rat DARPP-32. Reactivity with human DARPP-32 has not been tested.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PPP1R1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DARPP-32 Antibody (375604) [Unconjugated]

• DARPP32
• DARPP-32
• PPP1R1B
• protein phosphatase 1, regulatory (inhibitor) subunit 1B
• regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein that anomalously migrates at about
32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DARPP-32 Antibody (MAB4230)(17)

Publications using MAB4230

• Pelzel, RJ;Herbst, MA;Rozema, NB;Solem, MA;Gomez-Pastor, R; Silmitasertib, an FDA-designated orphan CK2 Inhibitor, ameliorates neuropathology and motor dysfunction in a Huntington's disease mouse model bioRxiv : the preprint server for biology 2025-11-20 [PMID: 41332649] (Immunohistochemistry, Western Blot, Mouse, Transgenic Mouse)
• Singh, V;Menard, MA;Serrano, GE;Beach, TG;Zhao, HT;Riley-DiPaolo, A;Subrahmanian, N;LaVoie, MJ;Volpicelli-Daley, LA; Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brain Acta neuropathologica communications 2023-12-18 [PMID: 38110990] (Immunohistochemistry, Mouse)
• Mansky RH, Greguske EA, Yu D et al. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease Cell reports 3/2/2023 [PMID: 36867535]
• L Duvick, WM Southern, K Benzow, HP Handler, JS Mitchell, H Kuivinen, UK Gadiparthi, P Yang, A Soles, C Scheeler, O Rainwater, S Shannah, E Larson, T Nichols-Me, Y You, B Oâ Callagh, HY Zoghbi, JM Ervasti, M Cvetanovic, MD Koob, HT Orr Regional vulnerability in a neurodegenerative disease: Delineating SCA1 CNS and muscle therapeutic targets using a conditional mutant ATXN1 mouse bioRxiv : the preprint server for biology, 2023-04-11;0(0):. 2023-04-11 [PMID: 36798410] (Western Blot, Mouse)
• Colleen S. Stein, Jared M. McLendon, Nathan H. Witmer, Ryan L. Boudreau Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson’s disease Molecular Therapy - Nucleic Acids 6/14/2022 [PMID: 35280925]
• M Derbis, E Kul, D Niewiadoms, M Sekrecki, A Piasecka, K Taylor, RK Hukema, O Stork, K Sobczak Short antisense oligonucleotides alleviate the pleiotropic toxicity of RNA harboring expanded CGG repeats Nature Communications, 2021-02-24;12(1):1265. 2021-02-24 [PMID: 33627639] (Western Blot, Mouse)
• ME Schmidt, NS Caron, AE Aly, FL Lemarié, L Dal Cengio, Y Ko, N Lazic, L Anderson, B Nguyen, LA Raymond, MR Hayden DAPK1 Promotes Extrasynaptic GluN2B Phosphorylation and Striatal Spine Instability in the YAC128 Mouse Model of Huntington Disease Front Cell Neurosci, 2020-11-05;14(0):590569. 2020-11-05 [PMID: 33250715] (ICC, Mouse)
• Machiela E, Jeloka R, Caron NS et al. The interaction of aging and oxidative stress contributes to pathogenesis in mouse and human Huntington disease neurons Front Aging Neurosci 11/16/2020 [PMID: 33192449]
• Paulina Carriba, Sean Wyatt, Alun M. Davies CD40L Reverse Signaling Influences Dendrite Spine Morphology and Expression of PSD-95 and Rho Small GTPases Frontiers in Cell and Developmental Biology 4/28/2020 [PMID: 32411702]
• CJ Burrus, SU McKinstry, N Kim, MI Ozlu, AV Santoki, FY Fang, A Ma, YB Karadeniz, AK Worthingto, I Dragatsis, S Zeitlin, HH Yin, C Eroglu Striatal Projection Neurons Require Huntingtin for Synaptic Connectivity and Survival Cell Rep, 2020-01-21;30(3):642-657.e6. 2020-01-21 [PMID: 31968243] (IHC, Mouse)
• NS Caron, AL Southwell, CC Brouwers, LD Cengio, Y Xie, HF Black, LM Anderson, S Ko, X Zhu, SJ van Devent, MM Evers, P Konstantin, MR Hayden Potent and sustained huntingtin lowering via AAV5 encoding miRNA preserves striatal volume and cognitive function in a humanized mouse model of Huntington disease Nucleic Acids Res., 2020-01-10;0(0):. 2020-01-10 [PMID: 31745548] (IHC, Mouse)
• ME Schmidt, C Buren, JP Mackay, D Cheung, L Dal Cengio, LA Raymond, MR Hayden Altering cortical input unmasks synaptic phenotypes in the YAC128 cortico-striatal co-culture model of Huntington disease BMC Biol., 2018-06-27;16(1):58. 2018-06-27 [PMID: 29945611] (ICC, Mouse)
• R Gomez-Past, ET Burchfiel, DW Neef, AM Jaeger, E Cabiscol, SU McKinstry, A Doss, A Aballay, DC Lo, SS Akimov, CA Ross, C Eroglu, DJ Thiele Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington's disease Nat Commun, 2017-02-13;8(0):14405. 2017-02-13 [PMID: 28194040] (IHC, Mouse)
• Yehong Wan, Kristen K. Ade, Zachary Caffall, M. Ilcim Ilcim Ozlu, Cagla Eroglu, Guoping Feng et al. Circuit-Selective Striatal Synaptic Dysfunction in the Sapap3 Knockout Mouse Model of Obsessive-Compulsive Disorder Biological Psychiatry 2014-04-15 [PMID: 23414593] (Immunocytochemistry, Transgenic Mouse)
• Yehong Wan, Kristen K. Ade, Zachary Caffall, M. Ilcim Ilcim Ozlu, Cagla Eroglu, Guoping Feng et al. Circuit-Selective Striatal Synaptic Dysfunction in the Sapap3 Knockout Mouse Model of Obsessive-Compulsive Disorder Biological Psychiatry 4/15/2014 [PMID: 23414593]
• Chen , Meng, Wan , Yehong, Ade , Kristen, Ting , Jonathan, Feng , Guoping, Calakos , Nicole Sapap3 deletion anomalously activates short-term endocannabinoid-mediated synaptic plasticity. J Neurosci, 2011-06-29;31(26):9563-73. 2011-06-29 [PMID: 21715621] (IHC, Mouse)
• Teegarden SL, Scott AN, Bale TL Early life exposure to a high fat diet promotes long-term changes in dietary preferences and central reward signaling. Neuroscience, 2009-05-22;162(4):924-32. 2009-05-22 [PMID: 19465087] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PPP1R1B
• Uniprot:
  • Q9UD71.2()