PDE10A Antibody

Images

 
Western Blot: PDE10A Antibody [NB300-645] - Rat Striatum: 1. Pan-PDE10A antibody. 2. PDE10A2 variant selective antibody. Diluted 1:5000in DiluOBuffer.
Immunohistochemistry-Paraffin: PDE10A Antibody - 1:1000 dilution in IHC Block Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X mangnification.FFPE section. Mouse Brain.
Western Blot: PDE10A Antibody [NB300-645] - NB300-645 (1:500) using CNS Blot. 1. Frontal Cortex 2. Pariental Cortex 3. Striatum 4. Amygdela 5. Hypothalamus 6. Hippocampus 7. Olfactory bulb 8. Brain Stem 9. Cerebellum ...read more
Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645] - Slide A is negative control. Slide B is stained with NB300-645 at 1:200 antibody dilution in DiluObuffer.

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.7 mg/ml

Order Details

PDE10A Antibody Summary

Immunogen
Synthetic peptide taken within amino acid region 300-350 on human PDE10A2 protein common to all PDE10A variants.
Localization
Located mostly to soluble cellular fractions.
Specificity
This has no cross-reactivity against any other PDE family members tested so far.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDE10A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot > 1:500
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation > 1:250
Application Notes
Immunocytochemistry (PMID: 21559457), Detects a band of approximately 90-93 kDa in rat brain and rat pancreas samples. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Control
Brain Lysate (NB820-59705)
Pancreas Lysate (NB820-59713)
PDE10A Lysate (NBL1-14213)
Publications
Read Publications using
NB300-645 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer, pH 7.4; cryoprotective agents; HEPES; stabilizing protein (BSA 0.5%), and glycerol (30%).
Preservative
0.02% Sodium Azide
Concentration
0.7 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDE10A Antibody

  • cAMP and cAMP-inhibited cGMP 3'-5'-cyclic phosphodiesterase 10A
  • dJ416F21.1 (phosphodiesterase 10A)
  • EC 3.1.4
  • EC 3.1.4.17
  • EC 3.1.4.35
  • FLJ11894
  • FLJ25677
  • HSPDE10A
  • phosphodiesterase 10A
  • phosphodiesterase 10A1 (PDE10A1)

Background

One of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is PDE10. Only one member of the cAMP and cGMP-specific PDE10 family has been cloned and characterized. The PDE10A gene is expressed in at least two variant forms (PDE10A1, PDE10A2) generated by RNA splicing and/or the use of alternate initiation sites. PDE10A is widely expressed in the putamen and caudate nucleus in the brain, thyroid and testis. The expression of PDE10A is also abundant in Heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDE10A Antibody (NB300-645)(2)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(1)
All Applications
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Human
(1)
Rat
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDE10A Antibody (NB300-645). (Showing 1 - 1 of 1 FAQs).

  1. This website claims that Tian et al (2011) successfully used this Pde10A antibody. However, in this paper they say the antibody that they used is from Scottish Biomedical. Is the antibody that you are selling here the same as they used, just now available from Novus rather than Scottish Biomedical?
    • I pulled up that article and it in fact does cite Novus as the antibody source. If you read into the Immunocytochemistry section, or do a search on the article for the word Novus it will come up. I believe the Scottish Biomedical reference is for the BSA.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDE10A
Entrez
Uniprot