PDE10A Antibody

Western Blot: PDE10A Antibody [NB300-645] - Rat Striatum: 1. Pan-PDE10A antibody. 2. PDE10A2 variant selective antibody. Diluted 1:5000in DiluOBuffer.

Immunohistochemistry-Paraffin: PDE10A Antibody - 1:1000 dilution in IHC Block Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X mangnification.FFPE section. Mouse Brain.

Western Blot: PDE10A Antibody [NB300-645] - NB300-645 (1:500) using CNS Blot. 1. Frontal Cortex 2. Pariental Cortex 3. Striatum 4. Amygdela 5. Hypothalamus 6. Hippocampus 7. Olfactory bulb 8. Brain Stem 9. Cerebellum 10. Spinal cord and 11. Whole Brain.

Immunohistochemistry-Paraffin: PDE10A Antibody [NB300-645] - Slide A is negative control. Slide B is stained with NB300-645 at 1:200 antibody dilution in DiluObuffer.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.7 mg/ml

PDE10A Antibody Summary

• Description: Novus Biologicals Rabbit PDE10A Antibody (NB300-645) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. Anti-PDE10A Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide taken within amino acid region 300-350 on human PDE10A2 protein common to all PDE10A variants.
• Localization: Located mostly to soluble cellular fractions.
• Specificity: This has no cross-reactivity against any other PDE family members tested so far.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDE10A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation > 1:250
  • Western Blot > 1:500
• Application Notes: ICC/IF reactivity reported in (PMID: 21559457). Detects a band of approximately 90-93 kDa in rat brain and rat pancreas samples. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.7 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE10A Antibody

• cAMP and cAMP-inhibited cGMP 3'-5'-cyclic phosphodiesterase 10A
• dJ416F21.1 (phosphodiesterase 10A)
• EC 3.1.4
• EC 3.1.4.17
• EC 3.1.4.35
• FLJ11894
• FLJ25677
• HSPDE10A
• PDE10A
• phosphodiesterase 10A
• phosphodiesterase 10A1 (PDE10A1)

Background

One of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is PDE10. Only one member of the cAMP and cGMP-specific PDE10 family has been cloned and characterized. The PDE10A gene is expressed in at least two variant forms (PDE10A1, PDE10A2) generated by RNA splicing and/or the use of alternate initiation sites. PDE10A is widely expressed in the putamen and caudate nucleus in the brain, thyroid and testis. The expression of PDE10A is also abundant in Heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDE10A Antibody (NB300-645)(4)

Publications using NB300-645

• Lingfeng Luo, Yujun Cai, Yishuai Zhang, Chia G Hsu, Vyacheslav A Korshunov, Xiaochun Long, Peter A Knight, Bradford C Berk, Chen Yan Role of PDE10A in vascular smooth muscle cell hyperplasia and pathological vascular remodelling Cardiovascular Research 2022-08-01 [PMID: 34550322]
• Sugiyama K, Kuroiwa M, Shuto T et al. Subregion-specific regulation of dopamine D1 receptor signaling in the striatum: implication for L-DOPA-induced dyskinesia The Journal of Neuroscience 2021-06-15 [PMID: 34131032]
• Li N, Lee K, Xi Y et al. Phosphodiesterase 10A: a novel target for selective inhibition of colon tumor cell growth and beta-catenin-dependent TCF transcriptional activity. Oncogene 2014-04-07 [PMID: 24704829] (WB, IHC-P, Human)
• Tian X, Vroom C, Ghofrani H et al. Phosphodiesterase 10A upregulation contributes to pulmonary vascular remodeling PLoS One 2011-04-11 [PMID: 21494592] (ICC/IF, Rat)

FAQs for PDE10A Antibody (NB300-645). (Showing 1 - 1 of 1 FAQs).

1. This website claims that Tian et al (2011) successfully used this Pde10A antibody. However, in this paper they say the antibody that they used is from Scottish Biomedical. Is the antibody that you are selling here the same as they used, just now available from Novus rather than Scottish Biomedical?
   • I pulled up that article and it in fact does cite Novus as the antibody source. If you read into the Immunocytochemistry section, or do a search on the article for the word Novus it will come up. I believe the Scottish Biomedical reference is for the BSA.

Bioinformatics

• Gene Symbol: PDE10A
• Entrez:
  • 10846(Human)
  • 23984(Mouse)
  • 63885(Rat)
• Uniprot:
  • Q9Y233(Human)