PDCD6 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PDCD6 Polyclonal Antibody (Catalog # AF6015) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PDCD6 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PDCD6
  Met1-Val189
  Accession # AAI10292
• Specificity: Detects human, mouse, and rat PDCD in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PDCD6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDCD6 Antibody [Unconjugated]

• ALG2
• ALG-2
• Apoptosis-linked gene 2 protein
• apoptosis-linked gene-2
• FLJ46208
• MGC111017
• MGC119050
• MGC9123
• PDCD6
• PEF1B
• Probable calcium-binding protein ALG-2
• programmed cell death 6
• programmed cell death protein 6

Background

PDCD6 (Programmed cell death protein 6; also ALG-2 and apoptosis-linked gene 2 protein) is a 22 kDa member of the penta-EF hand protein family of molecules. It is ubiquitously expressed, and performs multiple functions. PDCD6 is contextually linked to apoptosis, proliferation, membrane fusion and vesicle trafficking. Human PDCD6 is 191 amino acids (aa) in length. Although it contains five EF-hand domains, only domains #1 and #3 are considered functional (aa 23-58 and 90‑125). There is one splice form (ALG-2.1) that shows a deletion of Gly121Phe122. This form typically represents about 1/3 of total cellular PDCD6. PDCD6/ALG-2 will homodimerize, and heterodimerize with ALG-2.1. Notably, there are classes of cytosolic proteins that bind PDCD6, but not ALG-2.1, accounting for differences in function. Both human PDCD6 isoforms share 99% aa identity with their mouse counterparts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD6
• Uniprot:
  • AAI10292()