Peflin Antibody - BSA Free

Western Blot: Peflin Antibody [NBP1-31613] - Sample(30 ug whole cell lysate)A:293T 12% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Peflin Antibody [NBP1-31613] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Peflin Antibody [NBP1-31613] - Paraffin-embedded SCC4 xenograft, using antibody at 1:100 dilution.

Western Blot: Peflin Antibody [NBP1-31613] - Demonstration of knockdown efficiencies and lack of Golgi disruption.(A) NRK cell lysates from the experiments documented in Fig 1 were immunoblotted to detect the Peflin indicated along the left edge of the image blocks. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27276012) licensed under a CC-BY license.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Peflin Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Peflin. The exact sequence is proprietary.
• Localization: Cytoplasm, Membrane, Peripheral membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PEF1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Peflin Antibody - BSA Free

• ABP32
• PEF protein with a long N-terminal hydrophobic domain
• PEF1A
• peflin
• penta-EF hand domain containing 1
• Penta-EF hand domain-containing protein 1
• penta-EF-hand domain containing 1

Background

PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpainsmall subunit (CAPNS1; MIM 114170), sorcin (SRI; MIM 182520), grancalcin (GCA; MIM 607030), and ALG2 (PDCD6; MIM601057) (Kitaura et al., 2001 (PubMed 11278427)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Peflin Antibody (NBP1-31613)(1)

Publication using NBP1-31613

• Rayl M, Truitt M, Held A et al. Penta-EF-Hand Protein Peflin Is a Negative Regulator of ER-To-Golgi Transport. PLoS One 2016-06-08 [PMID: 27276012] (WB)

Bioinformatics

• Gene Symbol: PEF1