PD-L2/B7-DC/PDCD1LG2 Antibody [Fluorescein]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse PD-L2/B7-DC Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1022F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

PD-L2/B7-DC/PDCD1LG2 Antibody [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PD-L2
  Leu20-Arg219
  Accession # Q9WUL5
• Specificity: Detects mouse PD-L2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human PD-L2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDCD1LG2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PD-L2/B7-DC/PDCD1LG2 Antibody [Fluorescein]

• B7-DC
• bA574F11.2
• Btdc
• Butyrophilin B7-DC
• Butyrophilin-like Protein
• CD273 antigen
• CD273
• CD273PD-1 ligand 2
• MGC142240
• PD-1-ligand 2
• PDCD1L2MGC142238
• PDCD1LG2
• PDL2
• PD-L2
• PDL2B7DC
• PD-L2PDCD1 ligand 2
• programmed cell death 1 ligand 2
• Programmed death ligand 2

Background

Mouse Programmed Death Ligand 2 (PD-L2), also named B7DC, CD273, and Butyrophilin-like Protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and B7-H3. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse PD-L2 cDNA encodes a 247 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 199 aa extracellular region containing one V-like and one C-like Ig domain, a 23 aa transmembrane region, and only a 5 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 72% aa sequence identity. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L1. Mouse PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. PD-L2 is constitutively expressed in lymphoid and non-lymphoid organs (1-4). The expression of PD-L2 is detected on endothelial cells, dendritic cells (DC), thymic epithelial cells, follicular, memory and regulatory B cells, macrophages and monocytes (1-5). PD-L2 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co-stimulatory function for the PD-1 ligands on resting T cells has also been reported. On DC, PD-L2 may promote a Th1 response, while on follicular B cells, PD-L2 would appear to regulate plasma cell and memory B cell generation (6,7).

1. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
2. Tseng, B.S-Y. et al. (2001) J. Exp. Med. 193:839.
3. Dai, S. et al. (2014) Cell Immunol. 290:72.
4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
5. Kamphorst, A.O. et al. (2013) Curr. Opin, Immunol. 25:381.
6. Singh, A.K. et al. (2011) Allergy 66:155.
7. Good-Jacobson, K.L. et al. (2010) Nat. Immunol. 11:535.
   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD1LG2
• Uniprot:
  • Q9WUL5()