PD-1 Antibody (J43) [PE]

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Flow Cytometry: PD-1 Antibody (J43) [PE] [NBP1-43700] - See notes for image caption

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
J43
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
PE
Concentration
0.2 mg/ml

PD-1 Antibody (J43) [PE] Summary

Immunogen
The immunogen for this antibody was PD1.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
PDCD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for PD-1 Antibody (J43) [PE]

  • CD279 antigen
  • CD279
  • hPD-1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2

Background

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PDCD1
Entrez