PCPTP1 Overexpression Lysate (Native)

Images

 
Western Blot: PCPTP1 Overexpression Lysate (Adult Normal) [NBL1-14987] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PCPTP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PCPTP1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC211122 and based on accession number NM_130846. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens protein tyrosine phosphatase, receptor type, R (PTPRR), transcript variant 2, mRNA.
Gene
PTPRR

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PCPTP1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PCPTP1 Overexpression Lysate (Native)

  • Ch-1 PTPase
  • ch-1PTPase
  • DKFZp781C1038
  • EC 3.1.3.48
  • ECPTP
  • EC-PTP
  • FLJ34328
  • MGC131968
  • MGC148170
  • NC-PTPCOM1
  • PCPTP1
  • protein tyrosine phosphatase Cr1PTPase
  • protein tyrosine phosphatase, receptor type, R
  • protein-tyrosine phosphatase NC-PTPCOM1
  • Protein-tyrosine phosphatase PCPTP1
  • PTPBR7
  • PTPRQ
  • PTP-SL
  • receptor-type tyrosine-protein phosphatase R
  • R-PTP-R

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domains, and thus represents a receptor-type PTP. The similar gene predominately expressed in mouse brain was found to associate with, and thus regulate the activity and cellular localization of MAP kinases. The rat counterpart of this gene was reported to be regulated by the nerve growth factor, which suggested the function of this gene in neuronal growth and differentiation. [provided by RefSeq]. Transcript Variant: This variant (2) contains a different 5' end region, and uses a downstream in-frame start codon, when compared to variant 1. The resulting protein lacks N-terminal 245 aa, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB7475
Species: Hu, Rt
Applications: WB
AF4937
Species: Hu
Applications: WB, IHC
H00005250-B02P
Species: Hu
Applications: WB, ICC/IF
NBP2-01340
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-47833
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NB500-138
Species: Hu
Applications: WB, IHC, IHC-P
NLS6792
Species: Hu, Mu, Po, Bt, Bv, Ca, Eq, Ha, Mk, Pm, Rb
Applications: IHC-P
AF1347
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NB300-202
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
NBP1-71770
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF932
Species: Hu
Applications: WB, Simple Western, IHC, ICC

Publications for PCPTP1 Lysate (NBL1-14987) (0)

There are no publications for PCPTP1 Lysate (NBL1-14987).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PCPTP1 Lysate (NBL1-14987) (0)

There are no reviews for PCPTP1 Lysate (NBL1-14987). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PCPTP1 Lysate (NBL1-14987) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PCPTP1 Products

Bioinformatics Tool for PCPTP1 Lysate (NBL1-14987)

Discover related pathways, diseases and genes to PCPTP1 Lysate (NBL1-14987). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PCPTP1 Lysate (NBL1-14987)

Discover more about diseases related to PCPTP1 Lysate (NBL1-14987).
 

Pathways for PCPTP1 Lysate (NBL1-14987)

View related products by pathway.

PTMs for PCPTP1 Lysate (NBL1-14987)

Learn more about PTMs related to PCPTP1 Lysate (NBL1-14987).
 

Research Areas for PCPTP1 Lysate (NBL1-14987)

Find related products by research area.

Blogs on PCPTP1

There are no specific blogs for PCPTP1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

PCPTP1 Antibody
NBP1-84912

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PCPTP1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PTPRR