PTPN7 Antibody (OTI3B10)

Western Blot: PTPN7 Antibody (3B10) [NBP2-01340] Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN7 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: PTPN7 Antibody (3B10) [NBP2-01340] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN7.

Western Blot: PTPN7 Antibody (3B10) [NBP2-01340] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN7.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ICC/IF
• Clone: OTI3B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.72 mg/ml

PTPN7 Antibody (OTI3B10) Summary

• Immunogen: Full length human recombinant protein of human PTPN7(NP_542155) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPN7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 44.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.72 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTPN7 Antibody (OTI3B10)

• BPTP-4
• FLJ18703
• HEPTPnonreceptor-type, stress induced
• LPTP
• protein tyrosine phosphatase, non-receptor type 7
• PTPN7
• tyrosine-protein phosphatase non-receptor type 7

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for PTPN7 Antibody (NBP2-01340) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Agbolahan Sofela WB Human 04/11/2019

Summary

• Application: Western Blot
• Sample Tested: Tissue Lysates
• Species: Human
• Lot: A001

Bioinformatics

• Gene Symbol: PTPN7
• Entrez:
  • 5778(Human)