PCK1 Antibody - BSA Free

Western Blot: PCK1 Antibody [NBP1-80928] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80928] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PCK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PCK1 Antibody - BSA Free (NBP1-80928) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KVVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQRDTVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  PCK1 Protein (NBP1-80928PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PCK1 Antibody - BSA Free

• EC 4.1.1.32
• MGC22652
• PCK1
• PEPCK1
• PEPCKC
• PEPCK-C
• PEPCK-CPEP carboxykinase
• phosphoenolpyruvate carboxykinase 1 (soluble)
• phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• phosphoenolpyruvate carboxykinase, cytosolic
• Phosphoenolpyruvate carboxylase
• phosphopyruvate carboxylase

Background

The PCK1 gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expressio

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCK1
• Entrez:
  • 5105(Human)
• Uniprot:
  • P35558(Human)