PCGF1 Antibody - BSA Free

Immunohistochemistry-Paraffin: PCGF1 Antibody [NBP1-82767] - Staining in human testis and pancreas tissues using anti-PCGF1 antibody. Corresponding PCGF1 RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: PCGF1 Antibody [NBP1-82767] - Staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: PCGF1 Antibody [NBP1-82767] - Staining of human testis shows high expression.

Immunohistochemistry-Paraffin: PCGF1 Antibody [NBP1-82767] - Staining of human pancreas shows low expression as expected.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PCGF1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LVPGLQDSEEKRIREFYQSRGLDRVTQPTGEEPALSNLGLPFSSFDHSKAHYYRYDEQLNLCLERLSSGKDKNKSVLQNKYVRCSVRAEVRHLRRVLCHRLMLNPQHVQLLFDNEVLPDHMTMKQIWLSRWFGKPSPLLLQYSVK
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCGF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PCGF1 Protein (NBP1-82767PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PCGF1 Antibody - BSA Free

• MGC10882
• Nervous system Polycomb-1
• NSPc1
• NSPC1FLJ43754
• polycomb group ring finger 1,2010002K04Rik
• polycomb group RING finger protein 1
• RING finger protein 68
• RNF68RNF3A-2

Background

PCGF1 is a mammalian homolog of the Drosophila polycomb group genes, which act as transcriptional repressors toregulate anterior-posterior patterning in early embryonic development (Nunes et al., 2001 (PubMed 11287196)). See alsoPCGF2 (MIM 600346).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCGF1