PBK Antibody - BSA Free

Western Blot: PBK Antibody [NBP2-58043] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human testis using Anti-PBK antibody NBP2-58043.

Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human endometrium shows low expression as expected.

Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human testis shows high expression.

Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining in human testis and endometrium tissues using anti-PBK antibody. Corresponding PBK RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human colon, kidney, liver and testis using Anti-PBK antibody NBP2-58043 (A) shows similar protein distribution across tissues to independent antibody NBP1-84342 (B).

Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human kidney.

Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human colon.

Immunohistochemistry-Paraffin: PBK Antibody [NBP2-58043] - Staining of human liver.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PBK Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PBK
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  PBK Recombinant Protein Antigen (NBP2-58043PEP)

Reactivity Notes

Mouse 84%, Rat 88%

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PBK Antibody - BSA Free

• Cancer/testis antigen 84
• CT84MAPKK-like protein kinase
• EC 2.7.12.2
• FLJ14385
• Nori-3PDZ-binding kinase
• PDZ binding kinase
• serine/threonine protein kinase
• Spermatogenesis-related protein kinase
• SPKT-LAK cell-originated protein kinase
• TOPKlymphokine-activated killer T-cell-originated protein kinase

Background

PBK encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBK