Pax7 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Pax7 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Pax7
  Met1-Gly196
  Accession # P23759
• Specificity: Detects human Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Pax3 is observed and approximately 4% cross-reactivity with rhPax1 and rhPax6 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax7 Antibody [Alexa Fluor® 488]

• FLJ37460
• HUP1
• paired box 7
• paired box gene 7
• paired box homeotic gene 7
• paired box protein Pax-7
• paired domain gene 7
• PAX7 transcriptional factor
• Pax7
• PAX7B
• RMS2

Background

PAX7 (Paired box 7A; also HuP1) is a 57 kDa member of the paired homeobox family of transcription factors. It is expressed in select neurons (in rodent) and skeletal muscle satellite cells, where, in the latter, it promotes both a muscle stem cell state (PAX7+ desmin- cells) and a lineage commitment state (PAX7+ desmin+ cells). PAX7 forms heterodimers with PAX3, and interacts with the regulatory regions of the Myf5 locus. Human PAX7(A) is 520 amino acids (aa) in length. It contains an N‑terminal paired box DNA-binding domain (aa 34-163), a homeodomain (aa 218-275) and a C-terminal poly-alanine region (aa 340-346). Multiple splice forms of PAX7 exist. There is a 54 kDa isoform B that contains a 38 aa substitution for aa 468-520, plus a second isoform that shows a deletion of Gly151 and Leu152. There is also a chromosomal rearrangement that fuses aa 30-385 of PAX7 to aa 211-265 of FOXO1A/FKHR. Over aa 1-196, human PAX7 shares 86% and 96% aa identity with human PAX3 and mouse PAX7, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.