PATZ Antibody (1B2) - Azide and BSA Free

Western Blot: PATZ Antibody (1B2) [H00023598-M01] - Analysis of PATZ1 expression in transfected 293T cell line by PATZ1 monoclonal antibody (M01), clone 1B2.Lane 1: PATZ1 transfected lysate (Predicted MW: 57.6 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: PATZ Antibody (1B2) [H00023598-M01] - Detection limit for recombinant GST tagged PATZ1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PATZ Antibody (1B2) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PATZ Antibody (1B2) - Azide and BSA Free (H00023598-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PATZ1 (NP_114440.1, 260 a.a. ~ 358 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLTGKRGRGRPRKANLLDSMFGSPGGLREAGILPCGLCGKVFTDANRLRQHEAQHGVTSLQLGYIDLPPPRLGENGLPISEDPDGPRKRSRTRKQVACE
• Specificity: Reacts with POZ (BTB) and AT hook containing zinc finger 1.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PATZ1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in western blot and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PATZ Antibody (1B2) - Azide and BSA Free

• dJ400N23
• MAZRPOZ-, AT hook-, and zinc finger-containing protein 1
• PATZZinc finger and BTB domain-containing protein 19
• POZ (BTB) and AT hook containing zinc finger 1
• Protein kinase A RI subunit alpha-associated protein
• RIAZBTB/POZ domain zinc finger transcription factor
• ZBTB19BTB-POZ domain zinc finger transcription factor
• Zinc finger protein 278Zinc finger sarcoma gene protein
• ZNF278POZ-AT hook-zinc finger protein
• ZSGMAZ-related factor

Background

The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PATZ1
• Entrez:
  • 23598(Human)
• Uniprot:
  • NP_114440(Human)