PARP4 Antibody

Product Discontinued

PARP4 Antibody Summary

• Immunogen: Synthetic peptide, DTHELKQKRTDC, representing the internal region of the human protein according to NP_006428.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PARP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:16000
  • Western Blot 0.3 ug/ml
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave an approx 80-85kDa band in Human Tonsil and HeLa lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 193kDa according to NP_006428.1. The 80-85kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reactivity Notes

Human, Mouse, Rat and Dog.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for PARP4 Antibody

• 193 kDa vault protein
• ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1
• ADPRTL1
• ADPRTL1I-alpha-I-related
• ARTD4
• EC 2.4.2.30
• H5 proline-rich
• KIAA0177
• p193
• PARP4
• PARP-4
• PARPL
• PARP-related
• PARP-related/IalphaI-related H5/proline-rich
• PH5P
• poly (ADP-ribose) polymerase family, member 4
• poly [ADP-ribose] polymerase 4
• poly(ADP-ribose) synthetase
• poly(ADP-ribosyl)transferase-like 1
• Vault poly(ADP-ribose) polymerase
• vault protein, 193-kDa
• VAULT3
• von Willebrand factor A domain containing 5C
• VPARP
• VPARPKIAA0177
• VWA5C

Background

Component of the vault ribonucleoprotein particle which is at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PARP4
• Entrez:
  • 143(Human)
  • 328417(Mouse)
  • 361046(Rat)
• Uniprot:
  • NP_006428.1(Human)