PARN Antibody - BSA Free

Immunohistochemistry-Paraffin: PARN Antibody [NBP1-84303] - Staining in human testis and pancreas tissues using anti-PARN antibody. Corresponding PARN RNA-seq data are presented for the same tissues.

Western Blot: PARN Antibody [NBP1-84303] - Analysis using Anti-PARN antibody NBP1-84303 (A) shows similar pattern to independent antibody NBP1-84302 (B).

Immunohistochemistry-Paraffin: PARN Antibody [NBP1-84303] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: PARN Antibody [NBP1-84303] - Staining of human testis shows high expression.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PARN Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QEELNDAVGFSRVIHAIANSGKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELEKRLKETPFNPPKVESAEGFPSYD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PARN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  PARN Protein (NBP1-84303PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PARN Antibody - BSA Free

• DANEC 3.1.13.4
• Deadenylating nuclease
• Deadenylation nuclease
• poly(A)-specific ribonuclease (deadenylation nuclease)
• poly(A)-specific ribonuclease PARN
• Polyadenylate-specific ribonuclease

Background

Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. The amino acid sequence of poly(A)-specific ribonuclease shows homology to the RNase D family of 3'-exonucleases. The protein appears to be localized in both the nucleus and the cytoplasm. It is not stably associated with polysomes or ribosomal subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PARN