pan Actin Antibody (ACTN05 (C4))

Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - EHD2 localizes to the plasma membrane independent of its interaction partners, syndapin2 and EHBP1. HeLa cells were treated with non-targeting control-, syndapin2- or EHBP1-siRNA for 72 h. Cells were then lysed, and equal amounts of proteins were separated by SDS-PAGE and subjected to immunoblotting with antibodies against syndapin2, EHBP1, EHD2, caveolin1 or actin. The immunoblots are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074519) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Human microvascular endothelial cells stained with pan Actin antibody (green) and Von Willebrand Factor (red) antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of Actin pan showing positive staining in the cytoplasm of Human prostate carcinoma (right) compared with a negative control in the absence of primary antibody (left).

Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of Actin in MCF-7 and HCT116 cell lysates (20ug/lane). Image from verified customer review.

Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Immunoprecipitation and Western Blot analaysis on pan Actin from Human Cells.

Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of HeLa Cells.

Western Blot: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of 25ug of various whole cell lysates and 10ul of PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-HCl polyacrylamide gel.

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of Actin pan (green) showing staining in the cytoplasm of T-47D cells (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of Actin pan (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of Actin pan (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of actin (green) in COS7 cells.

Immunohistochemistry-Paraffin: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis showing positive staining in the cytoplasm of Mouse skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).

Immunohistochemistry-Paraffin: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis showing positive staining in the cytoplasm of Human skeletal muscle (right) compared with a negative control in the absence of primary antibody (left).

Immunoprecipitation: pan Actin Antibody (ACTN05 (C4)) [NB600-535] - Analysis of denatured Human HeLa Cells.

Product Discontinued

pan Actin Antibody (ACTN05 (C4)) Summary

• Immunogen: Chicken gizzard actin
• Localization: Cytoplasmic
• Specificity: This antibody reacts with all six known vertebrate isoforms of actin (approximately 42kDa). It also reacts with two cytoplasmic actins (Beta, gamma) which are highly homologous to one another but which differ from the muscle actins at about 25 amino acid residues.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:200
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2 ug
  • Western Blot 1:2500 - 1:10000
• Application Notes: WB: Detects a 42 kDa protein.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  HeLa Whole Cell Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for pan Actin Antibody (ACTN05 (C4))

• ACTA
• actin, alpha 1, skeletal muscle
• alpha skeletal muscle actin
• alpha skeletal muscle
• alpha-actin-1
• ASMA
• CFTD
• CFTDM
• MPFD
• NEM1
• NEM2
• NEM3

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for pan Actin Antibody (NB600-535)(21)

Publications using NB600-535

• Xie S, Naslavsky N, Caplan S EHD1 promotes CP110 ubiquitination by centriolar satellite delivery of HERC2 to the mother centriole EMBO reports 2023-04-19 [PMID: 37074924] (WB, Human)
• Christensen S, Narimatsu Y, Simoes S et al. Endosomal trafficking is required for glycosylation and normal maturation of the Alzheimers-associated protein sorLA bioRxiv 2020-07-14 (WB, KO, Mouse)
• Angelini A, Trial J, Saltzman AB et al. A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart iScience 2023-08-18 [PMID: 37520701]
• Luedeman M, Stroik S, Feng W et al. Poly(ADP) ribose polymerase promotes DNA polymerase theta-mediated end joining by activation of end resection Nat Commun 2022-08-04 [PMID: 35927262]
• Bhattacharjee S, Rehman I, Basu S et al. Interplay between symmetric arginine dimethylation and ubiquitylation regulates TDP1 proteostasis for the repair of topoisomerase I-DNA adducts Cell reports 2022-06-14 [PMID: 35705029]
• Simoes S, Guo J, Buitrago L et al. Alzheimer's vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling Cell reports 2021-12-28 [PMID: 34965419] (WB, Human, Mouse)
• Simoes S, Neufeld J. L, et al. Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans. Sci Transl Med 2020-11-25 [PMID: 33239387]
• Maidorn M, Olichon A, Rizzoli SO, Opazo F. Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons MAbs 2018-11-22 [PMID: 30466346] (WB, Human)
• Richter Katharina N, Wildhagen Hanna, Helm Martin S et al. Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins. Scientific Reports 2018-10-04 [PMID: 30287847] (Rat)
• Qureshi YH, Patel VM, Berman DE et al. An Alzheimer's linked loss-of-function CLN5 variant impairs Cathepsin D maturation consistent with a retromer trafficking defect. Mol. Cell. Biol. 2018-07-23 [PMID: 30037983] (WB, Mouse)
• Williamson RL, Laulagnier K, Miranda AM et al. Disruption of amyloid precursor protein ubiquitination selectively increases amyloid beta (Ab) 40 levels via presenilin 2-mediated cleavage. J. Biol. Chem. 2017-10-11 [PMID: 29021256]
• Xu F, Wang Y, Tao T et al. Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization. Cell Stress Chaperones 2017-01-01 [PMID: 27815707] (Human)
• Safdar A, Khrapko K, Flynn JM et al. Exercise-induced mitochondrial p53 repairs mtDNA mutations in mutator mice. Skelet Muscle 2015-01-01 [PMID: 26834962] (WB, Mouse)
• Simpson DA, Lemonie N, Morgan DS et al. Oncogenic BRAF(V600E) Induces Clastogenesis and UVB Hypersensitivity. Cancers (Basel) 2015-01-01 [PMID: 26091525] (WB)
• Xie S, Naslavsky N, Caplan S. Diacylglycerol kinase alpha regulates tubular recycling endosome biogenesis and major histocompatibility complex class I recycling. J Biol Chem. 2014-09-23 [PMID: 25248744]
• Wilhelm B G, Mandad S et al. Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins. Science 2014-05-30 [PMID: 24876496] (ICC/IF, WB, Rat)
• Simone LC, Caplan S, Naslavsky N. Role of Phosphatidylinositol 4,5-Bisphosphate in Regulating EHD2 Plasma Membrane Localization. PLoS One. 2013-09-10 [PMID: 24040268] (WB, Human)
• Pietri M, Dakowski C, Hannaoui S et al. PDK1 decreases TACE-mediated alpha-secretase activity and promotes disease progression in prion and Alzheimer's diseases. Nat Med. 2013-09-01 [PMID: 23955714] (WB, Mouse)
• Michalak Z, Lebrun A et al. IgG leakage may contribute to neuronal dysfunction in drug-refractory epilepsies with blood-brain barrier disruption. J Neuropathol Exp Neurol 2012-01-09 [PMID: 22878666] (WB, Rat)
• Hijikata T, Nakamura A, Isokawa K et al. Plectin 1 links intermediate filaments to costameric sarcolemma through {beta}-synemin, {alpha}-dystrobrevin actin. J Cell Sci;121(12):2062-2074. 2008-01-01 [PMID: 18505798]
• Lessard J. Two Monoclonal antibodies to actin: One muscle reactive one generally reactive. Cell Motility Cytoskeleton 10: 349- 362. 1988-01-01 [PMID: 2460261]

Reviews for pan Actin Antibody (NB600-535) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Christian Zuppinger ICC Human 07/14/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: cultured cells
• Species: Human
• Lot: OJ1766921

reviewed by: Verified Customer WB Human 10/16/2014

Summary

• Application: Western Blot
• Sample Tested: Cell lysate from MCF7 and HCT116 cells grown in DMEM medium
• Species: Human

Bioinformatics

• Gene Symbol: ACTA1
• Entrez:
  • 58(Human)
  • 60(Human)
  • 71(Human)
  • 11459(Mouse)
  • 29437(Rat)