Pallidin Antibody

Western Blot: Pallidin Antibody [NB100-1234] - HEK293 overexpressing PLDN and probed with (mock transfection in first lane).

Western Blot: Pallidin Antibody [NB100-1234] - (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Pallidin Antibody Summary

• Description: Novus Biologicals Goat Pallidin Antibody (NB100-1234) is a polyclonal antibody validated for use in WB and ELISA. Anti-Pallidin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EREKQLTARPAKRM corresponding to C-Terminus according to NP_036520.1.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BLOC1S6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 23-24 kDa band observed in A431 lysates (calculated MW of 19.7 kDa band according to NP_036520.1). In transfected HEK293 transiently expressing PLDN a band of approx. 28 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  Pallidin Overexpression Lysate
  A-431 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pallidin Antibody

• pallid (mouse) homolog, pallidin
• Pallid protein homolog
• PALLID
• pallidin homolog (mouse)
• pallidin
• PAsyntaxin 13 binding protein 1
• syntaxin 13-interacting protein pallid
• Syntaxin 13-interacting protein

Background

Pallidin, encoded by gene HGNC, may play a role in intracellular vesicle trafficking. It interacts with Syntaxin 13 which mediates intracellular membrane fusion. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pallidin Antibody (NB100-1234)(2)

Publications using NB100-1234

• Kouloumenta A, Mavroidis M, Capetanaki Y. Proper perinuclear localization of the TRIM-like protein myospryn requires its binding partner desmin J Biol Chem. 2007-11-30 [PMID: 17872945] (WB, Mouse)
• Huang L, Kuo YM, Gitschier J. The pallid gene encodes a novel, syntaxin 13-interacting protein involved in platelet storage pool deficiency. Nat Genet 1999-11-01 [PMID: 10610180]

Bioinformatics

• Gene Symbol: BLOC1S6
• Entrez:
  • 26258(Human)
• Uniprot:
  • NP_036520.(Human)