PAK7 Antibody - BSA Free

Western Blot: PAK7 Antibody [NBP1-77237] - T24 lysate with PAK5 antibody at (A) 2 and (B) 4 u/mL.

Immunocytochemistry/Immunofluorescence: PAK7 Antibody [NBP1-77237] - K562 cells with PAK5 antibody at 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: PAK7 Antibody - BSA Free [NBP1-77237] - Immunofluorescence of PAK7 in K562 cells with PAK7 antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PAK7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PAK7 Antibody - BSA Free (NBP1-77237) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-PAK7 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK5. The immunogen is located within amino acids 240 - 290 of PAK5. Amino Acid Squence: GPSLDDYDRRPKS
• Predicted Species: Mouse (100%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAK5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Western Blot 2-4 ug/ml
• Control Peptide:

  PAK7 Protein (NBP1-77237PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PAK7 Antibody - BSA Free

• PAK5
• PAK7

Background

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK5 was initially identified as a kinase expressed primarily in brain that while possessing a kinase domain and GTPase binding domain similar to PAK4 and PAK6, is completely different from both. Expression of PAK5 in neural based cell lines resulted in neurite outgrowth suggesting that PAK5 may be involved in regulating the cytoskeletal changes necessary for promoting neurite outgrowth. Other experiments suggest that unlike the other PAKs, PAK5 may inhibit apoptosis by phosphorylating the Bcl-2 family member Bad.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAK7 Antibody (NBP1-77237)(1)

Publication using NBP1-77237

• Huang N, Li S, Xie Y et al. Reprogramming an energetic AKT-PAK5 axis boosts axon energy supply and facilitates neuron survival and regeneration after injury and ischemia Current biology : CB 2021-05-26 [PMID: 34087103]

Bioinformatics

• Gene Symbol: PAK5
• Uniprot:
  • Q9P286()