PADI4 Antibody

Western Blot: PADI4 Antibody [NBP3-18652] - Figure 2. Western blot analysis using the antibody directed against PADI4 Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 ug) with the antibody against PADI4 , diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

ELISA: PADI4 Antibody [NBP3-18652] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against PADI4 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PADI4 Antibody Summary

• Description: Novus Biologicals Rabbit PADI4 Antibody (NBP3-18652) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PADI4
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.05% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for PADI4 Antibody

• HL-60 PAD
• PAD
• PAD4
• PADI4
• PADI5PADI-H protein
• PADPAD4
• PDI4
• PDI5EC 3.5.3.15
• peptidyl arginine deiminase, type IV
• peptidyl arginine deiminase, type V
• Peptidylarginine deiminase IV
• Protein-arginine deiminase type IV
• protein-arginine deiminase type-4

Background

In vivo expression of PADI4 correlated with the appearance of citrulline in histones. We have shown that PADI4 converts arginines in the histone H3 tail to citrulline, that PADI4 blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to eostrogen regulated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PADI4
• Uniprot:
  • Q9UM07(Human)