PADI4 Antibody (OTI5C10)

Western Blot: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.

Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Human Ovary tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.

Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI5C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PADI4 Antibody (OTI5C10) Summary

• Description: Novus Biologicals Mouse PADI4 Antibody (OTI5C10) (NBP2-46310) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment correspongding to amino acids 299-588 of human PADI4(NP_036519) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PADI4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 73.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PADI4 Antibody (OTI5C10)

• HL-60 PAD
• PAD
• PAD4
• PADI4
• PADI5PADI-H protein
• PADPAD4
• PDI4
• PDI5EC 3.5.3.15
• peptidyl arginine deiminase, type IV
• peptidyl arginine deiminase, type V
• Peptidylarginine deiminase IV
• Protein-arginine deiminase type IV
• protein-arginine deiminase type-4

Background

In vivo expression of PADI4 correlated with the appearance of citrulline in histones. We have shown that PADI4 converts arginines in the histone H3 tail to citrulline, that PADI4 blocks transcriptional activation and is recruited to the endogenous promotor. PADI4 represents a novel class of histone modifying enzymes probably linked to eostrogen regulated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PADI4