PACSIN3 Antibody - BSA Free

Western Blot: PACSIN3 Antibody [NBP2-47339] - Analysis using Anti-PACSIN3 antibody NBP2-47339 (A) shows similar pattern to independent antibody NBP2-48707 (B).

Immunocytochemistry/Immunofluorescence: PACSIN3 Antibody [NBP2-47339] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Immunohistochemistry-Paraffin: PACSIN3 Antibody [NBP2-47339] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal and glial cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PACSIN3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: KPWLKRLKEVEASKKSYHAARKDEKTAQTRESHAKADSAVSQEQLRKLQERVERCAKEAEKTKAQYEQTLAELHR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PACSIN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  PACSIN3 Protein (NBP2-47339PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PACSIN3 Antibody - BSA Free

• Endophilin I
• FLJ17861
• protein kinase C and casein kinase substrate in neurons 3
• protein kinase C and casein kinase substrate in neurons protein 3
• SDPIII
• SH3 domain-containing protein 6511
• syndapin III

Background

PACSIN3 (protein kinase C and casein kinase substrate in neurons protein 3) is a member of the PACSIN family, a group of adaptor protein that regulate vesicle trafficking via cytoskeletal alterations. PACSIN3 is primarily expressed in skeletal muscle and heart and has been shown to be specifically involved in the trafficking of GLUT 1 in adipocytes. PACSIN3 is also known as SH3 domain-containing 6511, syndapin III, and SDPIII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PACSIN3