p97/VCP Antibody (JM11-15)

Western Blot: p97/VCP Antibody (JM11-15) [NBP2-67008] - Analysis of VCP on hybrid fish (crucian-carp) heart tissue lysate using anti-VCP antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (JM11-15) [NBP2-67008] - Staining VCP in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: p97/VCP Antibody (JM11-15) [NBP2-67008] - Analysis of paraffin-embedded rat brain tissue using anti-VCP antibody. Counter stained with hematoxylin.

Flow Cytometry: p97/VCP Antibody (JM11-15) [NBP2-67008] - Flow cytometric analysis of HL-60 cells with p97/VCP antibody at 1/50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: p97/VCP Antibody (JM11-15) [NBP2-67008] - Analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution. Positive control: Line 1: SH-SY5Y Line 2: HepG2 Line 3: Hela

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (JM11-15) [NBP2-67008] - Staining VCP in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (JM11-15) [NBP2-67008] - Staining VCP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: p97/VCP Antibody (JM11-15) [NBP2-67008] - Analysis of paraffin-embedded human breast cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: p97/VCP Antibody (JM11-15) [NBP2-67008] - Analysis of paraffin-embedded human colon cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: p97/VCP Antibody (JM11-15) [NBP2-67008] - Analysis of paraffin-embedded human liver tissue using anti-VCP antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: p97/VCP Antibody (JM11-15) [NBP2-67008] - Analysis of paraffin-embedded mouse colon tissue using anti-VCP antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: p97/VCP Antibody (JM11-15) [NBP2-67008] - Analysis of paraffin-embedded zebrafish tissue using anti-VCP antibody. Counter stained with hematoxylin.

Product Discontinued

p97/VCP Antibody (JM11-15) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human p97/VCP aa 1-48 / 806. (SwissProt: P55072 Human; SwissProt: Q01853 Mouse; SwissProt: P46462 Rat)
• Localization: Cytoplasm, Endoplasmic reticulum, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VCP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for p97/VCP Antibody (JM11-15)

• 15S Mg(2+)-ATPase p97 subunit
• IBMPFD
• IBMPFDMGC8560
• MGC131997
• p97
• TER ATPase
• TERA
• transitional endoplasmic reticulum ATPase
• valosin containing protein
• Valosin-containing proteinMGC148092
• VCP
• yeast Cdc48p homolog

Background

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VCP