p97/VCP Antibody (5)

Images

 
Western Blot: p97/VCP Antibody (5) [NB120-11433] - Analysis of CA46 cell lysate.
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (5) [NB120-11433] - Analysis of VCP using VCP Monoclonal antibody (5) shows staining in C6 glioma cells. VCP staining (green), F-Actin staining with Phalloidin ...read more
Immunohistochemistry-Paraffin: p97/VCP Antibody (5) [NB120-11433] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (5) [NB120-11433] - Analysis of VCP using VCP Monoclonal antibody (5) shows staining in WiDr colon carcinoma cells. VCP staining (green), F-Actin staining with ...read more
Immunocytochemistry/ Immunofluorescence: p97/VCP Antibody (5) [NB120-11433] - VCP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and ...read more
Immunohistochemistry-Paraffin: p97/VCP Antibody (5) [NB120-11433] - Both normal and cancer biopsies of deparaffinized Human brain tissue tissues.

Product Details

Summary
Reactivity Hu, Mu, Rt, YeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

p97/VCP Antibody (5) Summary

Immunogen
Synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP.
Localization
Cytoplasmic and Nuclear
Specificity
This detects VCP protein in human, mouse, and rat samples.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
VCP
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:400
  • Immunocytochemistry/Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 97 kDa protein.
Positive Control
p97/VCP Lysate (NBL1-17707)
Reviewed Applications
Read 1 Review rated 3
using
NB120-11433 in the following applications:

Publications
Read Publication using
NB120-11433 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for p97/VCP Antibody (5)

  • 15S Mg(2+)-ATPase p97 subunit
  • IBMPFD
  • IBMPFDMGC8560
  • MGC131997
  • p97
  • TER ATPase
  • TERA
  • transitional endoplasmic reticulum ATPase
  • valosin containing protein
  • Valosin-containing proteinMGC148092
  • VCP
  • yeast Cdc48p homolog

Background

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p97/VCP Antibody (NB120-11433)(1)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for p97/VCP Antibody (NB120-11433) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using NB120-11433:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot p97/VCP NB120-11433
Enlarge
reviewed by:
Anonymous
WB Human and Mouse 06/29/2017
View

Summary

ApplicationWestern Blot
Sample Tested293FT cell lysate,nih3t3 cell lysate
SpeciesHuman and Mouse
Lotrh237363

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p97/VCP Antibody (NB120-11433) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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3
1
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1
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Anonymous
06/29/2017
Application: WB
Species: Human and Mouse

Bioinformatics

Gene Symbol VCP
Entrez