p53 [p Ser15] Antibody - BSA Free

Western Blot: p53 [p Ser15] Antibody [NB100-1913] - Analysis of extract from UV treated (lane 2) and untreated (Lane 1) HeLa cells.

Product Discontinued

p53 [p Ser15] Antibody - BSA Free Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab1431)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human p53.
• Modification: p Ser15
• Specificity: The phospho-p53 (Ser15) detects ~53 kDa phosphorylated p53 at Ser15. It does not react with non-phosphorylated p53 or p53 phosphorylated at other sites.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP53
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:200
  • Immunoprecipitation 1:10 - 1:100
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  p53 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.42% Potassium phosphate (pH 7.3), 0.87% Sodium chloride, 30% glycerol
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for p53 [p Ser15] Antibody - BSA Free

• Antigen NY-CO-13
• BCC7
• FLJ92943
• LFS1
• LFS1TRP53
• p53 tumor suppressor
• p53
• P53cellular tumor antigen p53
• Phosphoprotein p53
• TP53
• transformation-related protein 53
• TRP53
• tumor protein p53
• Tumor suppressor p53

Background

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p53 Antibody (NB100-1913)(2)

Publications using NB100-1913

• Bernard JJ, Lou YR, Peng QY et al. Inverse relationship between p53 and phospho-Chk1 (Ser317) protein expression in UVB-induced skin tumors in SKH-1 mice. Exp Mol Pathol. 2013-10-30 [PMID: 24184701] (WB, Mouse)
• Shangary, S et al. Lyn regulates the cell death response to ultraviolet radiation through c-Jun terminal Kinase-dependent Fas lig activation. Experimental Cell Research 0:1-10. 2003-01-01 [PMID: 12941605]

FAQs for p53 Antibody (NB100-1913). (Showing 1 - 1 of 1 FAQs).

1. This product is powder or liquid? Could you suggest me the concentration for human sample (IHC-P)?
   • I am showing that this product comes in liquid form at 0.5 mg/mL and the suggested concentration for IHC is 20 ug/mL. This of course is just a guide and must be optimized by you based on how highly expressed the protein is in your samples.

Bioinformatics

• Gene Symbol: TP53
• Entrez:
  • 7157(Human)
  • 22059(Mouse)
  • 24842(Rat)
• Uniprot:
  • P04637()