p23/PTGES3 Antibody

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody [NBP2-19998] - Mouse testis. p23 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: p23/PTGES3 Antibody [NBP2-19998] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:10000

Western Blot: p23/PTGES3 Antibody [NBP2-19998] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:5000

Wild-type (WT) and p23 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with p23 antibody [N1C3] (NBP2-19998) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Pm, RM
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

p23/PTGES3 Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit p23/PTGES3 Antibody (NBP2-19998) is a polyclonal antibody validated for use in IHC and WB. Anti-p23/PTGES3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human p23/PTGES3. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Monkey (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTGES3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:20000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Bovine reported in secitific publication PMID: 32861111

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for p23/PTGES3 Antibody

• CPGES
• cPGESp23
• Cytosolic prostaglandin E2 synthase
• Hsp90 co-chaperone
• p23
• P23cytosolic prostaglandin E synthase
• Progesterone receptor complex p23
• prostaglandin E synthase 3 (cytosolic)
• prostaglandin E synthase 3
• PTGES3
• TEBP
• TEBPEC 5.3.99.3
• Telomerase-binding protein p23
• unactive progesterone receptor, 23 kD

Background

Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for p23/PTGES3 Antibody (NBP2-19998)(1)

Publication using NBP2-19998

• Qin X, Yang S, Zhang Y et al. Effects of non-esterified fatty acids on relative abundance of prostaglandin E2 and F2 alpha synthesis-related mRNA transcripts and protein in endometrial cells of cattle in vitro Animal Reproduction Science 2020-10-01 [PMID: 32861111] (WB, Bovine)

Bioinformatics

• Gene Symbol: PTGES3
• Uniprot:
  • Q15185()