p21/CIP1/CDKN1A Antibody (SR1330) - BSA Free

Western Blot: p21/CIP1/CDKN1A Antibody (SR1330) [NBP3-21676] - Western blot analysis using NBP3-21676 in (1) MCF-7 cell lysate; (2) HeLa cell lysate. (3) LnCap cell lysate; (4) U87 MG cell lysate.

Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (SR1330) [NBP3-21676] - Immunofluorescent analysis of MCF7 cells, using NBP3-21676.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: SR1330
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

p21/CIP1/CDKN1A Antibody (SR1330) - BSA Free Summary

• Description: Novus Biologicals Rabbit p21/CIP1/CDKN1A Antibody (SR1330) - BSA Free (NBP3-21676) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human p21/CIP1/CDKN1A (Uniprot #: P38936)
• Specificity: Detects endogenous levels of total p21/CIP1/CDKN1A
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDKN1A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for p21/CIP1/CDKN1A Antibody (SR1330) - BSA Free

• CAP20cyclin-dependent kinase inhibitor 1
• CDK-interacting protein 1
• CDKN1A
• CDKN1melanoma differentiation associated protein 6
• CIP1
• CIP1WAF1CDK-interaction protein 1
• cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• MDA6
• MDA-6
• Melanoma differentiation-associated protein 6
• p21
• p21CIP1
• p21Cip1/Waf1
• PIC1
• SDI1DNA synthesis inhibitor
• wild-type p53-activated fragment 1

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN1A